Mechanical Performance and Cytotoxicity of an Alginate/Polyacrylamide Bipolymer Network Developed for Medical Applications

Abstract

Several hydrogels could be used as scaffolds for tissue engineering and a model of extracellular matrices for biological studies. However, the scope of alginate in medical applications is often severely limited by its mechanical behavior. In the present study, the modification of the mechanical properties of the alginate scaffold is obtained by its combination with polyacrylamide in order to obtain a multifunctional biomaterial. The advantage of this double polymer network is due to an improvement in the mechanical strength with regard to the alginate alone, and in particular, its Young’s modulus values. The morphological study of this network was carried out by scanning electron microscopy (SEM). The swelling properties were also studied over several time intervals. In addition to mechanical property requirements, these polymers must meet several biosafety parameters as part of an overall risk management strategy. Our preliminary study illustrates that the mechanical property of this synthetic scaffold depends on the ratio of the two polymers (alginate, polyacrylamide) which allows us to choose the appropriate ratio to mimic replaceable body tissue and be used in various biological and medical uses, including 3D cell culture, tissue engineering, and protection against local shocks.