Abstract
Ten jatrophane diterpenoids were isolated from the whole plant Euphorbia peplus Linn. including seven new ones, named euphjatrophanes A-G (labeled compounds 1, 2, 4–8). Their structures were elucidated with a combination of spectroscopic and single-crystal X-ray crystallography, enabling the identification of compounds 3, 9, and 10 as the previously published euphpepluones G, K, and L, respectively. All compounds were evaluated for their bioactivity with flow cytometry in assays of autophagic flux in HM Cherry-GFP-LC3 (human microglia cells stably expressing the tandem monomeric mCherry-GFP-tagged LC3) cells. Euphpepluone K (9) significantly activated autophagic flux, an effect that was verified with confocal analysis. Moreover, cellular assays showed that euphpepluone K (9) induced autophagy and inhibited Tau pathology.
Funder
National Natural Science Foundation of China
Yunnan Provincial Science and Technology Department
Guizhou Provincial Science and Technology projects
Original Innovation Project “from 0 to 1” of the Basic Frontier Scientific Research Program, CAS
Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China
Scientific and Technological Foundation of Guizhou Health Committee
Cultivation Project of National Natural Science Foundation from Guizhou Medical University
Basic Research Program of Yunnan Province
Youth Innovation Promotion Association of CAS
CAS “Light of West China” Program
Young Scientific and Technological Talents Promotion Project of the Yunnan Association for Science and Technology
National Key R&D Program of China
Open Project from Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Cited by
3 articles.
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