DDR1 and Its Ligand, Collagen IV, Are Involved in In Vitro Oligodendrocyte Maturation
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Published:2023-01-16
Issue:2
Volume:24
Page:1742
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Silva Maria Elena,
Hernández-Andrade Matías,
Abasolo Nerea,
Espinoza-Cruells Cristóbal,
Mansilla Josselyne B.,
Reyes Carolina R.ORCID,
Aranda Selena,
Esteban Yaiza,
Rodriguez-Calvo RicardoORCID,
Martorell LourdesORCID,
Muntané Gerard,
Rivera Francisco J.,
Vilella ElisabetORCID
Abstract
Discoidin domain receptor 1 (DDR1) is a tyrosine kinase receptor expressed in epithelial cells from different tissues in which collagen binding activates pleiotropic functions. In the brain, DDR1 is mainly expressed in oligodendrocytes (OLs), the function of which is unclear. Whether collagen can activate DDR1 in OLs has not been studied. Here, we assessed the expression of DDR1 during in vitro OL differentiation, including collagen IV incubation, and the capability of collagen IV to induce DDR1 phosphorylation. Experiments were performed using two in vitro models of OL differentiation: OLs derived from adult rat neural stem cells (NSCs) and the HOG16 human oligodendroglial cell line. Immunocytofluorescence, western blotting, and ELISA were performed to analyze these questions. The differentiation of OLs from NSCs was addressed using oligodendrocyte transcription factor 2 (Olig2) and myelin basic protein (MBP). In HOG16 OLs, collagen IV induced DDR1 phosphorylation through slow and sustained kinetics. In NSC-derived OLs, DDR1 was found in a high proportion of differentiating cells (MBP+/Olig2+), but its protein expression was decreased in later stages. The addition of collagen IV did not change the number of DDR1+/MBP+ cells but did accelerate OL branching. Here, we provide the first demonstration that collagen IV mediates the phosphorylation of DDR1 in HOG16 cells and that the in vitro co-expression of DDR1 and MBP is associated with accelerated branching during the differentiation of primary OLs.
Funder
Spanish Instituto de Salud Carlos III from the Ministry of Economy, Industry and Competitivity and FEDER
Generalitat de Catalunya Universities Agency
Chilean FONDECYT Program ANID
PCI program ANID
Chilean FONDEF-IDeA Program
Universitat Rovira i Virgili
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Cited by
1 articles.
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