Numerical Simulation of Bubble-Liquid Two-Phase Turbulent Flows in Shallow Bioreactor

Abstract

An improved second-order moment bubble-liquid two-phase turbulent model is developed to predict the hydrodynamic characteristics of the shallow bioreactor using two height-to-diameter ratios of H/D = 1.4 and H/D = 2.9. The two-phase hydrodynamic parameters, the bubble normal and shear stress, the bubble energy dissipation rate, the bubble turbulent kinetic energy, etc. were numerically simulated. These parameters increased along with flow direction and constituted a threat to cells living at far distance away from the gas jetting inlet regions, rather than a finding of higher cell damage at near the jetting inlet region, as reported by Babosa et al. 2003. A new correlation named the turbulent energy production of bubble-liquid two-phase flow was proposed to successfully verify this experimental observation. A smaller H/D ratio makes more contributions to the generation of lower turbulent energy productions, which are in favor of the alleviation of cell damage. The extremely long and narrow shape of the bioreactor is deteriorative for cell living.