Exosomal miR-17-5p, miR-146a-3p, and miR-223-3p Correlate with Radiologic Sequelae in Survivors of COVID-19-Related Acute Respiratory Distress Syndrome
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Published:2023-08-22
Issue:17
Volume:24
Page:13037
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Curcio Rosa1, Poli Giulia2, Fabi Consuelo2, Sugoni Chiara2, Pasticci Maria Bruna23, Ferranti Roberto4, Rossi Monica4, Folletti Ilenia25ORCID, Sanesi Leandro1, Santoni Edoardo12, Dominioni Irene12, Cavallo Massimiliano1, Morgana Giovanni12, Mordeglia Lorenzo12ORCID, Luca Giovanni2, Pucci Giacomo12ORCID, Brancorsini Stefano2ORCID, Vaudo Gaetano12ORCID
Affiliation:
1. Unit of Internal Medicine, Santa Maria Terni Hospital, 05100 Terni, Italy 2. Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy 3. Infectious Diseases Unit, Santa Maria Terni Hospital, 05100 Terni, Italy 4. Unit of Radiology, Santa Maria Terni Hospital, 05100 Terni, Italy 5. Section of Occupational Medicine, Santa Maria Terni Hospital, 05100 Terni, Italy
Abstract
We investigated the association between circulating microRNAs (miRNAs) potentially involved in the lung inflammatory process and fibrosis development among COVID-19-related acute respiratory distress syndrome (ARDS) survivors. At 4 ± 2 months from clinical recovery, COVID-19-related ARDS survivors matched for age, sex, and clinical characteristics underwent chest high-resolution computerized tomography (HRCT) and were selected based on imaging pattern evolution into fully recovered (N = normal), pulmonary opacities (PO) and fibrosis-like lesions (FL). Based on the previous literature, we performed plasma miRNA profiling of exosomal miRNAs belonging to the NLRP3-inflammasome platform with validated (miR-17-5p, miR-223-3p) and putative targets (miR-146a-5p), miRNAs involved in the post-transcriptional regulation of acute phase cytokines (miR128-3p, miR3168, miR125b-2-3p, miR106a-5p), miRNAs belonging to the NLRP4-inflammasome platform (miR-141-3p) and miRNAs related to post-transcriptional regulation of the fibrosis process (miR-21-5p). miR-17-5p, miR-223-3p, and miR-146a-5p were significantly down-regulated in patients with FL when compared to patients with PO. miR-146a-5p was also down-regulated in patients with FL than in N. The expression of the remaining miRNAs did not differ by group. In patients with long-term pulmonary radiological sequelae following COVID-19-related ARDS, a down-regulation of miR-17-5p, miR-146a-3p, and miR-223-3p correlated to fibrosis development in patients showing persistent hyper-reactivity to inflammatory stimulation. Our results support the hypothesis that NLRP3-Inflammasome could be implicated in the process of fibrotic evolution of COVID-19-associated ARDS.
Funder
University of Perugia, Italy
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Cited by
1 articles.
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