Effects of Aging on Collagen in the Skeletal Muscle of Mice

Author:

Kanazawa Yuji1ORCID,Miyachi Ryo1,Higuchi Takashi2,Sato Hiaki3

Affiliation:

1. Department of Physical Therapy, Hokuriku University, Kanazawa 920-1180, Ishikawa, Japan

2. Department of Physical Therapy, Osaka University of Human Sciences, Settsu 566-8501, Osaka, Japan

3. Department of Medical Technology and Clinical Engineering, Hokuriku University, Kanazawa 920-1180, Ishikawa, Japan

Abstract

Aging affects several tissues in the body, including skeletal muscle. Multiple types of collagens are localized in the skeletal muscle and contribute to the maintenance of normal muscle structure and function. Since the effects of aging on muscle fibers vary by muscle fiber type, it is expected that the effects of aging on intramuscular collagen might be influenced by muscle fiber type. In this study, we examined the effect of aging on collagen levels in the soleus (slow-twitch muscle) and gastrocnemius (fast-twitch muscle) muscles of 3-, 10-, 24-, and 28-month-old male C57BL/6J mice using molecular and morphological analysis. It was found that aging increased collagen I, III, and VI gene expression and immunoreactivity in both slow- and fast-twitch muscles and collagen IV expression in slow-twitch muscles. However, collagen IV gene expression and immunoreactivity in fast-twitch muscle were unaffected by aging. In contrast, the expression of the collagen synthesis marker heat shock protein 47 in both slow- and fast-twitch muscles decreased with aging, while the expression of collagen degradation markers increased with aging. Overall, these results suggest that collagen gene expression and immunoreactivity are influenced by muscle fiber type and collagen type and that the balance between collagen synthesis and degradation tends to tilt toward degradation with aging.

Funder

Japan Society for the Promotion of Science (JSPS) KAKENHI

Hokuriku University Special Research

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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