Affiliation:
1. Molecular Pathology, Department of Translational Medicine, Skåne University Hospital, Lund University, 21428 Malmö, Sweden
Abstract
The centrosome of mammalian cells is in constant movement and its motion plays a part in cell differentiation and cell division. The purpose of this study was to establish the involvement of the TUBG meshwork in centrosomal motility. In live cells, we used a monomeric red-fluorescence-protein-tagged centrin 2 gene and a green-fluorescence-protein-tagged TUBG1 gene for labeling the centrosome and the TUBG1 meshwork, respectively. We found that centrosome movements occurred in cellular sites rich in GTPase TUBG1 and single-guide RNA mediated a reduction in the expression of TUBG1, altering the motility pattern of centrosomes. We propose that the TUBG1 meshwork enables the centrosomes to move by providing them with an interacting platform that mediates positional changes. These findings uncover a novel regulatory mechanism that controls the behavior of centrosomes.
Funder
Swedish Cancer Society
Swedish Childhood Cancer Foundation
Skane University Hospital in Malmö Cancer Research Fund
Lund University, Sweden
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference49 articles.
1. Towards a molecular architecture of centriole assembly;Gonczy;Nat. Rev. Mol. Cell Biol.,2012
2. The chromosome cycle and the centrosome cycle in the mitotic cycle;Mazia;Int. Rev. Cytol.,1987
3. The centrosome is an actin-organizing centre;Farina;Nat. Cell Biol.,2016
4. Microtubule nucleation by gamma-tubulin complexes;Kollman;Nat. Rev. Mol. Cell Biol.,2011
5. Zhou, J., Corvaisier, M., Malycheva, D., and Alvarado-Kristensson, M. (2022). Hubbing the Cancer Cell. Cancers, 14.