Differential Epigenetic Changes in the Dorsal Hippocampus of Male and Female SAMP8 Mice: A Preliminary Study

Author:

Ravanelli Federico1,Musazzi Laura2ORCID,Barbieri Silvia Stella3ORCID,Rovati Gianenrico1ORCID,Popoli Maurizio1ORCID,Barbon Alessandro4ORCID,Ieraci Alessandro5ORCID

Affiliation:

1. Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, Italy

2. Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy

3. Unit of Brain-Heart Axis: Cellular and Molecular Mechanisms, Centro Cardiologico Monzino IRCCS, 20138 Milan, Italy

4. Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy

5. Department of Theoretical and Applied Sciences, eCampus University, 22060 Novedrate, Italy

Abstract

Alzheimer’s disease (AD) is the most common age-related neurodegenerative disease characterized by memory loss and cognitive impairment. The causes of the disease are not well understood, as it involves a complex interaction between genetic, environmental, and epigenetic factors. SAMP8 mice have been proposed as a model for studying late-onset AD, since they show age-related learning and memory deficits as well as several features of AD pathogenesis. Epigenetic changes have been described in SAMP8 mice, although sex differences have never been evaluated. Here we used western blot and qPCR analyses to investigate whether epigenetic markers are differentially altered in the dorsal hippocampus, a region important for the regulation of learning and memory, of 9-month-old male and female SAMP8 mice. We found that H3Ac was selectively reduced in male SAMP8 mice compared to male SAMR1 control mice, but not in female mice, whereas H3K27me3 was reduced overall in SAMP8 mice. Moreover, the levels of HDAC2 and JmjD3 were increased, whereas the levels of HDAC4 and Dnmt3a were reduced in SAMP8 mice compared to SAMR1. In addition, levels of HDAC1 were reduced, whereas Utx and Jmjd3 were selectively increased in females compared to males. Although our results are preliminary, they suggest that epigenetic mechanisms in the dorsal hippocampus are differentially regulated in male and female SAMP8 mice.

Funder

Cariplo Foundation

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Reference66 articles.

1. Alzheimer’s Disease: Clinical Trials and Drug Development;Mangialasche;Lancet Neurol.,2010

2. Ageing as a Risk Factor for Neurodegenerative Disease;Hou;Nat. Rev. Neurol.,2019

3. Current Research Therapeutic Strategies for Alzheimer’s Disease Treatment;Folch;Neural Plast.,2016

4. Late-Onset Alzheimer Disease;Rabinovici;Continuum,2019

5. Epigenetic Modifications: Basic Mechanisms and Role in Cardiovascular Disease;Handy;Circulation,2011

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