Assembly and Annotation of the Complete Genome Sequence of the Paenibacillus Bacteriophage phJNUCC32

Author:

Xu Yang1,Liang Xuhui1,Hyun Chang-Gu1ORCID

Affiliation:

1. Department of Beauty and Cosmetology, Jeju Inside Agency and Cosmetic Science Center, Jeju National University, Jeju 63243, Republic of Korea

Abstract

A potential biocontrol agent for American foulbrood (AFB), the Paenibacillus bacteriophage phJNUCC32, was isolated from Baengnokdam in Halla Mountain. This study aimed to investigate its genomic characteristics through whole-genome sequencing. The genome of phJNUCC32 was found to be 62,871 base pairs in length, with a G + C content of 51.98%. Phylogenetic analysis classified phJNUCC32 within the unclassified Caudoviricetes bacteriophage category. The genome prediction confirmed the absence of virulence factors and antibiotic-resistance genes, ensuring its genetic safety. A total of 63 coding DNA sequences were identified, revealing a modular arrangement. Notably, the annotation of gene function indicates that phJNUCC32 harbors the holin/lysin system, suggesting significant potential for controlling bacterial infections in AFB and agriculture.

Funder

"Regional Innovation Strategy (RIS)" through the National Research Foundation of Korea funded by the Ministry of Education

Publisher

MDPI AG

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