Abstract
Hepatic steatosis signifies onset of metabolic dysfunction-associated fatty liver disease (MAFLD) caused by disrupted metabolic homeostasis compromising liver function. Regular consumption of common beans reduces the risk of metabolic impairment, but its effective dose, the impact of biological sex, and underlying mechanisms of action are unknown. We fed female and male C57BL6/J mice with obesogenic yet isocaloric diets containing 0%, 17.5%, 35%, and 70% of total dietary protein derived from cooked whole common beans. Liver tissue was collected for histopathology, lipid quantification, and RNA-seq analyses. Beans qualitatively and quantitatively diminished hepatic fat deposition at the 35% dose in female and 70% dose in male mice. Bean-induced differentially expressed genes (DEGs) most significantly mapped to hepatic steatosis and revealed dose-responsive inhibition of de novo lipogenesis markers (Acly, Acaca, Fasn, Elovl6, Scd1, etc.) and triacylglycerol biosynthesis, activation of triacylglycerol degradation, and downregulation of sterol regulatory element-binding transcription factor 1 (SREBF1) signaling. Upregulated fatty acid β-oxidation was more prominent in females, while suppression of Cd36-mediated fatty acid uptake—in males. Sex-dependent bean effects also involved DEGs patterns downstream of peroxisome proliferator-activated receptor α (PPARα) and MLX-interacting protein-like (MLXIPL). Therefore, biological sex determines amount of common bean in the diet required to prevent hepatic lipid accumulation.
Funder
U.S. Department of Agriculture, Agricultural Research Service
National Institute for Food and Agriculture
Subject
Food Science,Nutrition and Dietetics
Reference73 articles.
1. The importance of healthy dietary patterns in chronic disease prevention;Neuhouser;Nutr. Res.,2019
2. Metabolic syndrome pathophysiology and predisposing factors;Bovolini;Int. J. Sport. Med.,2021
3. Singh, R.B., Watanabe, S., and Isaza, A.A. (2022). Functional Foods and Nutraceuticals in Metabolic and Non-Communicable Diseases, Academic Press.
4. Dietary carbohydrates: Pathogenesis and potential therapeutic targets to obesity-associated metabolic syndrome;Akter;BioFactors,2022
5. Advancing the global public health agenda for NAFLD: A consensus statement;Lazarus;Nat. Rev. Gastroenterol. Hepatol.,2022
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献