Effect of Foliar Spraying of Gibberellins and Brassinolide on Cadmium Accumulation in Rice

Abstract

Cadmium (Cd) is one of the heavy metals that contaminate rice cultivation, and reducing Cd contamination in rice through agronomic measures is a hot research topic. In this study, foliar sprays of gibberellins (GA) and brassinolide (BR) were applied to rice under Cd stress in hydroponic and pot experiments. After foliar spraying of GR and BR, the biomass of rice plants grown in either hydroponics or soil culture was significantly higher or even exceeded that in the absence of Cd stress. In addition, photosynthetic parameters (maximum fluorescence values), root length and root surface area, and CAT, SOD and POD activities were significantly improved. The MDA content decreased in the shoots, suggesting that the application of GR and BA may have enhanced photosynthesis and antioxidant function to alleviate Cd stress. Furthermore, the BR and GA treatments decreased the Cd content of rice roots, shoots and grains as well as the Cd transfer coefficient. Cd chemical morphology analysis of rice roots and shoots showed that the proportion of soluble Cd (Ethanol-Cd and Water-Cd) decreased, whereas the proportion of NaCl-Cd increased. Analysis of the subcellular distribution of Cd in rice roots and above ground showed that the proportion of Cd in the cell wall increased after foliar spraying of GA and BR. The results indicate that after foliar application of GA and BR, more of the Cd in rice was transformed into immobile forms and was fixed in the cell wall, thus reducing the amount in the seeds. In summary, foliar sprays of GA and BR can reduce the toxic effects of Cd on rice plants and reduce the Cd content in rice grains, with GA being more effective.