Lysosomal Membrane Stability of Mussel (Mytilus galloprovincialis Lamarck, 1819) as a Biomarker of Cellular Stress for Environmental Contamination

Abstract

The lysosomal membrane stability (LMS) of hemocytes in wild mussels (Mytilus galloprovincialis) as a biomarker of cellular stress for chemical pollution was tested by neutral red retention time (NRRT) assays. To assess the environmental contamination in the study area, seawater quality and pollutant bioaccumulation throughout the soft tissue of mussels were investigated. The samples were collected in July 2022 at four sites on the Romanian Black Sea coast considered to be differently affected by contamination. To support the suitability of LMS as a biomarker of contaminant-induced stress, the contaminant body burdens of the mussels were compared with the NRRT values. The results showed a significantly reduced NRRT in all investigated locations, particularly in port areas (mean retention time between 11 and 14 min). The elevated bioaccumulation of organochlorinated pesticides (OCPs) and polychlorinated biphenyls (PCBs) and low NRRTs were observed at the most contaminated sites (i.e., ports). The low lysosomal stability reflected stress and damage in the hemocytes of mussels and could be related to the body burdens of contaminants. LMS is an effective indicator of health status in mussels and could be considered a sensitive biomarker of cellular stress induced by contaminant exposure.