Cortical Neurons Adjust the Action Potential Onset Features as a Function of Stimulus Type

Abstract

Pyramidal neurons and interneurons play critical roles in regulating the neuronal activities in the mammalian cortex, where they exhibit different firing patterns. Pyramidal neurons mainly exhibit regular-spiking firing patterns, while interneurons have fast-spiking firing patterns. Cortical neurons have distinct action potential onset dynamics, in which the evoked action potential is rapid and highly variable. However, it is still unclear how cortical regular-spiking and fast-spiking neurons discriminate between different types of stimuli by changing their action potential onset parameters. Thus, we used intracellular recordings of regular-spiking and fast-spiking neurons, taken from layer 2/3 in the somatosensory cortex of adult mice, to investigate changes in the action potential waveform in response to two distinct stimulation protocols: the conventional step-and-hold and frozen noise. The results show that the frozen noise stimulation paradigm evoked more rapid action potential with lower threshold potential in both neuron types. Nevertheless, the difference in the action potential rapidity in response to different stimuli was significant in regular-spiking pyramidal neurons while insignificant in fast-spiking interneurons. Furthermore, the threshold variation was significantly higher for regular-spiking neurons than for fast-spiking neurons. Our findings demonstrate that different types of cortical neurons exhibit various onset dynamics of the action potentials, implying that different mechanisms govern the initiation of action potentials across cortical neuron subtypes.