Characterization of Polyphenols and Volatile Compounds from Understudied Algerian Pallenis spinosa by HS-SPME-GC-MS, NMR and HPLC-MSn Approaches
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Published:2023-09-08
Issue:18
Volume:13
Page:10113
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ISSN:2076-3417
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Container-title:Applied Sciences
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language:en
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Short-container-title:Applied Sciences
Author:
Adoui Nabila1, Souilah Nabila2, Bendif Hamdi13ORCID, Sut Stefania4, Dall’Acqua Stefano4ORCID, Flamini Guido5ORCID, Maggi Filippo6ORCID, Peron Gregorio7ORCID
Affiliation:
1. Natural and Life Sciences Department, Faculty of Sciences, University of M’sila, BP 166, Msila 28000, Algeria 2. Department of Agronomic Sciences, Faculty of Sciences, University of Skikda, BP 26 Road of El Hadaiek, Skikda 21000, Algeria 3. Laboratory of Ethnobotany and Natural Substances, Department of Natural Sciences, Ecole Normale Superieure (ENS), BP 92 Kouba, Algiers 16308, Algeria 4. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35100 Padova, Italy 5. Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126 Pisa, Italy 6. School of Pharmacy, University of Camerino, Via S. Agostino 1, 62032 Camerino, Italy 7. Department of Molecular and Translational Medicine (DMMT), University of Brescia, Viale Europa 11, 25123 Brescia, Italy
Abstract
Pallenis spinosa (L.) Cass. is a widespread plant in the Mediterranean region. Traditionally, it is used as a medicinal species to treat several ailments, from inflammation to skin injuries. Although the phytochemical content of this plant has already been investigated, there is currently limited data on Algerian P. spinosa. In this work, we focused on volatile compounds and non-volatile secondary metabolites extracted using HS-SPME and methanol from the aerial parts of P. spinosa collected from Northeast Algeria. Volatile constituents were analyzed by GC-MS, while non-volatile compounds were analyzed by NMR and HPLC-MSn. In total, 48 volatile compounds were identified, including sesquiterpene hydrocarbons (65.8%), monoterpene hydrocarbons (16.9%), and oxygenated monoterpenes and sesquiterpenes (8.3% and 6.5%, respectively). β-Chamigrene (16.2%), α-selinene (12.8%), β-pinene (10.6%), and β-caryophyllene (9.2%) were assessed as the main constituents. Concerning non-volatile metabolites, 23 polyphenols were identified (7.26 mg/g DW), and phenolic acids were predominant (5.83 mg/g DW). Tricaffeoylhexaric acid (1.76 mg/g DW), tetracaffeoylhexaric acid (1.41 mg/g DW), 3,5-dicaffeoylquinic acid (1.04 mg/g DW), caffeoyl dihexoside (0.35 mg/g DW), and chlorogenic acid (0.29 mg/g DW) were the most abundant ones. Several known flavonoids, such as tricin and patuletin glycosides, kaempferol, and apigenin, were also identified, and myricetin hexoside was detected in P. spinosa for the first time. Overall, our work is the first to report an exhaustive characterization of volatile and non-volatile secondary metabolites from Algerian P. spinosa. The results represent a step forward in revealing the chemistry of this widespread plant species. Furthermore, they may contribute to rationalizing its traditional medicinal applications and preserve the biodiversity of Algerian flora.
Subject
Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science
Reference34 articles.
1. Eudesmane sesquiterpenoids from the Asteraceae family;Wu;Nat. Prod. Rep.,2006 2. Vegetation and species altitudinal distribution in Al-Jabal Al-Akhdar landscape, Libya;Hegazy;Pak. J. Bot,2011 3. Systematic and anatomical studies of Inuleae and Plucheeae in Egypt;Zareh;Feddes Repert.,2005 4. Studies on pharmaceutical ethnobotany in the region of Pallars (Pyrenees, Catalonia, Iberian Peninsula). Part II. New or very rare uses of previously known medicinal plants;Agelet;J. Ethnopharmacol.,2003 5. Chermat, S., Gharzouli, R., Quézel, P., and Santa, S. (2015). 1962–1963: Nouvelle flore de l’Algérie et des régions désertiques méridionales. Vol. 1, 2. CNRS, Paris, 566, 1170. J. Mater. Sci. Eng. A.
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