Fly-Ash Pollution Modulates Growth, Biochemical Attributes, Antioxidant Activity and Gene Expression in Pithecellobium Dulce (Roxb) Benth.

Abstract

This study investigates the effect of fly ash (FA) on the Pithecellobium dulce (Roxb) Benth. trees growing at three different locations. FA stress caused significant changes in different leaf attributes like sugar, protein contents, photosynthetic pigments, nitrate content and nitrate reductase activity in foliar tissues of plants growing at a highly contaminated site, as compared to a low-pollution site. Lower rates of stomatal conductance (SC) were observed in P. dulce leaves under fly ash stress conditions that drastically reduced net photosynthetic rate (PN); however, intercellular carbon dioxide concentration and stomatal index (SI) showed an increase under the same stress conditions. On the other hand, significant increase was also observed in the proline, sulphur and nitrogen contents. A significant increase in oxidative stress and, consequently, in antioxidant enzymes such as ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxidase dismutase (SOD) and Air pollution tolerance index were discovered at three different sites. The transcriptional expression of antioxidant and stress responsive genes was higher at HPS as compared to two other two sites of the study. Taken together the results demonstrated that the P. dulce is best suited as a fly ash stress tolerant plant species with the potential to provide an alternative for the reclamation of fly ash affected soils.