Composition Characterization of Crossostephium chinense Leaf Essential Oil and Its Anti-Inflammatory Activity Mechanisms

Author:

Lin Chia-Hsin12ORCID,Chiang Yu-Ting13,Lin Li-Yin3,Tsao Nai-Wen4ORCID,Wang Chung-Hsuan4ORCID,Chien Shih-Chang5,Sun Ying-Hsuan1ORCID,Wang Sheng-Yang146ORCID

Affiliation:

1. Department of Forestry, National Chung Hsing University, Taichung 402202, Taiwan

2. Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404328, Taiwan

3. Liyu International Co., Ltd., Taichung 402202, Taiwan

4. Special Crop and Metabolome Discipline Cluster, Academy Circle Economy, National Chung Hsing University, Taichung 402202, Taiwan

5. Experimental Forest Management Office, National Chung-Hsing University, Taichung 402202, Taiwan

6. Agricultural Biotechnology Research Center, Academia Sinica, Taipei City 115201, Taiwan

Abstract

This study investigates the composition characteristics and anti-inflammatory activity mechanisms of the essential oil from the leaves of Crossostephium chinense. C. chinense is a perennial herb commonly found in East Asia, traditionally used to treat various ailments. The essential oil extracted through water distillation, primarily contains 1,8-cineole (13.73%), santolina triene (13.53%), and germacrene D (10.67%). Three compounds were identified from the essential oil, namely 1-acetoxy-2-(2-hydroxypropyl)-5-methylhex-3,5-diene, 1-acetoxy-isopyliden-hex-5-en-4-one, and chrysanthemyl acetate, with the first two being newly discovered compounds. Then, the essential oil of C. chinense exhibits significant anti-inflammatory effects on RAW264.7 macrophages, effectively inhibiting the production of NO and ROS, with the IC50 value of 10.3 μg/mL. Furthermore, the essential oil reduces the expression of pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β. Mechanistic studies indicate that the essential oil affects the inflammatory response by inhibiting the expression of iNOS but has no significant impact on COX-2. Further analysis suggests that the essential oil may regulate the inflammatory response through the ERK protein in the MAPK pathway and IκBα in the NF-κB pathway, while also promoting the activity of the NRF2/HO-1 antioxidant pathway, enhancing the cell’s antioxidant capacity, thereby achieving an effect of inhibiting the inflammatory response. These results highlight the potential application value of C. chinense leaf essential oil in the medical and healthcare fields.

Funder

Ministry of Science and Technology

Publisher

MDPI AG

Reference25 articles.

1. Lee, D., Kwak, H.J., Kim, B.H., Kim, S.H., Kim, D.W., and Kang, K.S. (2021). Combined Anti-Adipogenic Effects of Hispidulin and p-Synephrine on 3T3-L1 Adipocytes. Biomolecules, 11.

2. Antioxidant and antiproliferative activities of Crossostephium chinensis (L.) Makino;Chang;Am. J. Chin. Med.,2009

3. Bioactivity guided isolation of alpha-glucosidase inhibitor from whole herbs of Crossostephium chinense;Wu;Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China J. Chin. Mater. Med.,2009

4. Wang, Z., Yamano, Y., Kawakami, S., Al-Hamoud, G.A., Sugimoto, S., Otsuka, H., and Matsunami, K. (2023). New ψ-Santonin Derivatives from Crossostephium chinense and Their Anti-Proliferative Activities against Leishmania major and Human Cancer Cells A549. Molecules, 28.

5. Further characterization of foliar flavonoids in Crossostephium chinense and their geographic variation;Uehara;Nat. Prod. Commun.,2014

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3