Application of Lavandula angustifolia Mill. Extracts for the Phytosynthesis of Silver Nanoparticles: Characterization and Biomedical Potential
Author:
Șuică-Bunghez Ioana Raluca1ORCID, Senin Raluca Mădălina1, Sorescu Ana Alexandra1, Ganciarov Mihaela1ORCID, Răut Iuliana1, Firincă Cristina12, Constantin Mariana13ORCID, Gifu Ioana Cătălina1ORCID, Stoica Rusăndica1, Fierăscu Irina14ORCID, Fierăscu Radu Claudiu15ORCID
Affiliation:
1. The National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM Bucharest, 202 Splaiul Independentei, 060021 Bucharest, Romania 2. Faculty of Biology, University of Bucharest, 91 Splaiul Independenței, 050104 Bucharest, Romania 3. Faculty of Pharmacy, “Titu Maiorescu” University, 187 Calea Vacaresti, 040051 Bucharest, Romania 4. Faculty of Horticulture, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania 5. Faculty of Chemical Engineering and Biotechnology, National University of Science and Technology Politehnica Bucharest, 1-7 Gheorghe Polizu St., 011061 Bucharest, Romania
Abstract
Nanotechnology can offer a series of new “green” and eco-friendly methods for developing different types of nanoparticles, among which the development of nanomaterials using plant extracts (phytosynthesis) represents one of the most promising areas of research. This present study details the use of lavender flowers (Lavandula angustifolia Mill., well-known for their use in homeopathic applications) for the biosynthesis of silver nanoparticles with enhanced antioxidant and antibacterial properties. Several qualitative and quantitative assays were carried out in order to offer an image of the extracts’ composition (the recorded total phenolics content varied between 21.0 to 40.9 mg GAE (gallic acid equivalents)/g dry weight (d.w.), while the total flavonoids content ranged between 3.57 and 16.8 mg CE (catechin equivalents)/g d.w.), alongside modern analytical methods (such as gas chromatography-mass spectrometry—GC-MS, quantifying 12 phytoconstituents present in the extracts). The formation of silver nanoparticles (AgNPs) using lavender extract was studied by UV-Vis spectroscopy, Fourier-transform infrared spectrometry (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and dynamic light scattering (DLS)/zeta potential, with the selected nanoparticles having crystallite sizes of approx. 14.55 nm (AgNP-L2) and 4.61 nm, respectively (for AgNP-L4), and hydrodynamic diameters of 392.4 nm (for AgNP-L2) and 391.6 nm (for AgNP-L4), determined by DLS. A zeta potential of around −6.4 mV was displayed for both samples while presenting as large aggregates, in which nanoparticle clusters with dimensions of around 130–200 nm can be observed. The biomedical applications of the extracts and the corresponding phytosynthesized nanoparticles were evaluated using antioxidant and antimicrobial assays. The obtained results confirmed the phytosynthesis of the silver nanoparticles using Lavandula angustifolia Mill. extracts, as well as their antioxidant and antimicrobial potential.
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
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