In Planta Detection of Beauveria bassiana (Ascomycota: Hypocreales) Strains as Endophytes in Bean (Phaseolus vulgaris L.)

Author:

Cavazos-Vallejo Teodora1,Valadez-Lira José Alberto1,Orozco-Flores Alonso A.1ORCID,Gomez-Flores Ricardo1,Ek-Ramos María Julissa1ORCID,Quistián-Martínez Deyanira2,Alcocer-González Juan Manuel1,Tamez-Guerra Patricia1ORCID

Affiliation:

1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas (FCB), Universidad Autónoma de Nuevo León (UANL), Avenida Pedro de Alba S/N, Ciudad Universitaria, San Nicolás de los Garza C.P. 66451, Nuevo León, Mexico

2. Departamento de Botánica, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Avenida Pedro de Alba S/N, Ciudad Universitaria, San Nicolás de los Garza C.P. 66451, Nuevo León, Mexico

Abstract

Beauveria bassiana (B. bassiana) is a significant entomopathogenic fungus (EPF) in agriculture as a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield. However, it is not known whether a B. bassiana strain has such a favorable impact on the plant, since it is a common soil microorganism. Therefore, techniques that allow strain monitoring will be advantageous. To date, methods for detecting or monitoring a specific EPF strain after external application are scarce. In the present study, an in planta nested PCR technique was standardized to differentiate between three B. bassiana strains (GHA, PTG4, and BB37) established as endophytes in bean plants under laboratory conditions by detecting the insertion profile of four group I introns located in the 28S gene of B. bassiana ribosomal DNA. This technique recognized a distinct pattern of bands of different sizes for each strain, with a sensitivity of 1 pg per 10 ng of plant DNA. This molecular approach may be more effective monitoring B. bassiana strains after application to evaluate their significance on crops.

Funder

CONAHCYT-MEXICO

Sistema Nacional de Investigadores e Investigadoras

Publisher

MDPI AG

Subject

Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics

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