Long-Lasting Silver Nanoparticles Synthesized with Tagetes erecta and Their Antibacterial Activity against Erwinia amylovora, a Serious Rosaceous Pathogen

Author:

Zarate-Escobedo Johana1ORCID,Zavaleta-Mancera Hilda Araceli2ORCID,Soto-Hernández Ramón Marcos2ORCID,Pérez-Rodríguez Paulino3ORCID,Vilchis-Nestor Alfredo Rafael4ORCID,Silva-Rojas Hilda Victoria5ORCID,Trejo-Téllez Libia Iris6ORCID

Affiliation:

1. Programa de Fisiología Vegetal, Colegio de Postgraduados en Ciencias Agrícolas Campus Montecillo, Montecillo, Texcoco 56264, Estado de México, Mexico

2. Programa de Botánica, Colegio de Postgraduados en Ciencias Agrícolas Campus Montecillo, Montecillo, Texcoco 56264, Estado de México, Mexico

3. Programa de Estadística, Colegio de Postgraduados en Ciencias Agrícolas Campus Montecillo, Montecillo, Texcoco 56264, Estado de México, Mexico

4. Centro Conjunto de Investigación en Química Sustentable, UAEM-UNAM, Toluca 50200, Estado de México, Mexico

5. Programa de Semillas, Colegio de Postgraduados en Ciencias Agrícolas Campus Montecillo, Montecillo, Texcoco 56264, Estado de México, Mexico

6. Laboratorio de Nutrición Vegetal, Programa de Edafología, Colegio de Postgraduados en Ciencias Agrícolas Campus Montecillo, Montecillo, Texcoco 56264, Estado de México, Mexico

Abstract

A rapid, eco-friendly, and simple method for the synthesis of long-lasting (2 years) silver nanoparticles (AgNPs) is reported using aqueous leaf and petal extracts of Tagetes erecta L. The particles were characterized using UV-Visible spectrophotometry and the analytical and crystallographic techniques of transmission electron microscopy (TEM). The longevity of the AgNPs was studied using UV-Vis and high-resolution TEM. The antibacterial activity of the particles against Erwinia amylovora was evaluated using the Kirby–Bauer disk diffusion method. The results were analyzed using ANOVA and Tukey’s test (p ≤ 0.05). Both the leaf and petal extracts produced AgNPs, but the leaf extract (1 mL) was long-lasting and quasi-spherical (17.64 ± 8.87 nm), with an absorbance of UV-Vis λmax 433 and a crystalline structure (fcc, 111). Phenols, flavonoids, tannins, and terpenoids which are associated with -OH, C=O, and C=C were identified in the extracts and could act as reducing and stabilizing agents. The best antibacterial activity was obtained with a nanoparticle concentration of 50 mg AgNPs L−1. The main contribution of the present research is to present a sustainable method for producing nanoparticles which are stable for 2 years and with antibacterial activity against E. amylovora, one of most threatening pathogens to pear and apple productions.

Funder

Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT) of Mexico

Publisher

MDPI AG

Reference73 articles.

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