Enhancing Resistance to Cercospora Leaf Spot in Mung Bean (Vigna radiata L.) through Bradyrhizobium sp. DOA9 Priming: Molecular Insights and Bio-Priming Potential
Author:
Songsaeng Apisit1ORCID, Boonchuen Pakpoom1, Nareephot Phongkeat1, Piromyou Pongdet2ORCID, Wongdee Jenjira2, Greetatorn Teerana2, Inthaisong Sukanya3, Tantasawat Piyada Alisha3ORCID, Teamtisong Kamonluck4, Tittabutr Panlada1ORCID, Sato Shusei5ORCID, Boonkerd Nantakorn1, Songwattana Pongpan2ORCID, Teaumroong Neung1ORCID
Affiliation:
1. School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand 2. Institute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand 3. School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand 4. The Center for Scientific and Technological Equipment, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand 5. Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
Abstract
Mung bean (Vigna radiata L.), a vital legume in Asia with significant nutritional benefits, is highly susceptible to Cercospora leaf spot (CLS) caused by Cercospora canescens, leading to significant yield losses. As an alternative to chemical fungicides, bio-priming with rhizobacteria can enhance plant resistance. This study explores the potential of Bradyrhizobium sp. strain DOA9 to augment resistance in mung bean against CLS via root priming. The results reveal that short (3 days) and double (17 and 3 days) priming with DOA9 before fungal infection considerably reduces lesion size on infected leaves by activating defense-related genes, including Pti1, Pti6, EDS1, NDR1, PR-1, PR-2, Prx, and CHS, or by suppressing the inhibition of PR-5 and enhancing peroxidase (POD) activity in leaves. Interestingly, the Type 3 secretion system (T3SS) of DOA9 may play a role in establishing resistance in V. radiata CN72. These findings suggest that DOA9 primes V. radiata CN72′s defense mechanisms, offering an effective bio-priming strategy to alleviate CLS. Hence, our insights propose the potential use of DOA9 as a bio-priming agent to manage CLS in V. radiata CN72, providing a sustainable alternative to chemical fungicide applications.
Funder
Suranaree University of Technology Thailand Science Research and Innovation National Science, Research, and Innovation Fund NSRF via the Program Management Unit for Human Resources & Institutional Development, Research, and Innovation JSPS-NRCT by National Research Council of Thailand The Office of the Permanent Secretary of the Ministry of Higher Education, Science, Research and Innovation
Reference80 articles.
1. Mung Bean: Technological and Nutritional Potential;Dahiya;Crit. Rev. Food Sci. Nutr.,2015 2. Hou, D., Yousaf, L., Xue, Y., Hu, J., Wu, J., Hu, X., Feng, N., and Shen, Q. (2019). Mung Bean (Vigna radiata L.): Bioactive Polyphenols, Polysaccharides, Peptides, and Health Benefits. Nutrients, 11. 3. Integrated Management against Root-Rot of Mungbean [Vigna radiata (L.) Wilczek] Incited by Macrophomina Phaseolina;Kumari;J. Plant Pathol. Microb.,2012 4. Sarkar, M., Datta, S., and Kundagrami, S. (2017). Global Climate Change and Mung Bean Production: A Roadmap towards Future Sustainable Agriculture. Sustaining Future Food Security in Changing Environment, Nova Science Publishers Inc. 5. Mungbean (Vigna radiata L. Wilczek) as Functional Food, Agronomic Importance and Breeding Approach for Development of Climate Resilience: Current Status and Future Perspectives;Sehrawat;Asian J. Biol. Life Sci.,2021
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