Potential of Drought Tolerant Rhizobacteria Amended with Biochar on Growth Promotion in Wheat

Abstract

Drought stress is the prime obstacle for worldwide agricultural production and necessitates innovative strategies for enhancing crop resilience. This study explores the efficacy of plant growth-promoting rhizobacteria (PGPR) and biochar (BC) as sustainable amendments for mitigating the effects of drought on wheat growth. Multiple experiments were carried out on isolated strains to assess their drought tolerance potential and multiple plant growth-promoting attributes. Experiments in the laboratory and natural environment were conducted to assess the impact of plant growth-promoting rhizobacteria, biochar, and their synergistic application on various growth parameters of wheat. The results revealed that the drought-tolerant PGPR strains (Bacillus subtilis and Bacillus tequilensis), alongside biochar (rice husk), alleviated the phytotoxic impact of drought by increasing the root length from 17.0% to 70.0% and shoot length from 30.0% to 82.0% as compared to un-inoculated stressed controls. The total chlorophyll and carotenoid contents of the plants were substantially increased to 477% and 423%, respectively, when biochar and PGPR were applied synergistically. Significant enhancements in membrane stability index, relative water content, proline, and sugar level were achieved by combining biochar and bacterial strains, resulting in increases of 19.5%, 37.9%, 219%, and 300%, respectively. The yield of wheat in terms of plant height, spike length, number of spikelets per spike, and number of grains per spike was enhanced from 26.7% to 44.6%, 23.5% to 62.7%, 91.5% to 154%, and 137% to 182%, respectively. It was concluded that the biochar-based application of PGPR induced drought tolerance in wheat under water deficit conditions, ultimately improving the production and yield of wheat.