Molecular Characterization Analysis and Adaptive Responses of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Nutritional and Enzymatic Variabilities in Various Maize Cultivars
Author:
Zhang Qiangyan1ORCID, Zhang Yanlei12, Zhang Kexin1ORCID, Liu Huiping1ORCID, Gou Yuping1ORCID, Li Chunchun1ORCID, Haq Inzamam Ul3ORCID, Quandahor Peter4ORCID, Liu Changzhong1ORCID
Affiliation:
1. Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China 2. Dingxi Plant Protection and Quarantine Station, Dingxi 743099, China 3. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China 4. CSIR—Savanna Agricultural Research Institute, Tamale P.O. Box 52, Ghana
Abstract
The fall armyworm, Spodoptera frugiperda Smith (Lepidoptera: Noctuidae), a common agricultural pest known for its extensive migration and wide host ranges, causes considerable harm to maize (Zea mays L.). In this study, we utilized two molecular marker genes, COI and Tpi, to compare the genetic characteristics of the collected original samples. Additionally, through an interactive study between S. frugiperda larvae and six maize varieties aiming to understand the insect’s adaptability and resistance mechanisms, our analysis revealed that both the COI and Tpi genes identified S. frugiperda as the corn strain. Further examination of the larvae showed significant differences in nutritional indices, digestive, and detoxification enzyme activities. Special maize varieties were found to offer higher efficiency in nutrient conversion and assimilation compared with common varieties. This study revealed adaptations in S. frugiperda’s digestive and detoxification processes in response to the different maize varieties. For instance, larvae reared on common maize exhibited elevated amylase and lipase activities. Interestingly, detoxification enzyme activities exhibited different patterns of variation in different maize varieties. The Pearson correlation analysis between nutritional indices, enzyme activities, and the nutritional content and secondary metabolites of maize leaves provided deeper insights into the pest’s adaptability. The results highlighted significant relationships between specific nutritional components in maize and the physiological responses of S. frugiperda. Overall, our findings contribute substantially to the understanding of S. frugiperda’s host plant adaptability, offering critical insights for the development of sustainable pest management strategies.
Funder
National Key Research and Development Program of China Science Project of Agriculture and Rural Department of Gansu Province Gansu Province excellent graduate “Innovation Star” project of China
Reference81 articles.
1. Goergen, G., Kumar, P.L., Sankung, S.B., Togola, A., and Tamò, M. (2016). First report of outbreaks of the fall armyworm Spodoptera frugiperda (JE Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in West and Central Africa. PLoS ONE, 11. 2. Otuka, A. (2023). Prediction of the Overseas Migration of the Fall Armyworm, Spodoptera frugiperda, to Japan. Insects, 14. 3. Advances in maize transformation technologies and development of transgenic maize;Yadava;Front. Plant Sci.,2017 4. Scott, P., Pratt, R.C., Hoffman, N., and Montgomery, R. (2019). Corn, Elsevier. 5. Host plants of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Americas;Montezano;Afr. Entomol.,2018
|
|