Affiliation:
1. College of Pharmacy, Xiangnan University, Chenzhou 423099, China
2. Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
3. College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
Abstract
Cadmium (Cd) is a highly toxic heavy metal that causes serious damage to plant and human health. Phytolacca acinosa Roxb. has a large amount of aboveground biomass and a rapid growth rate, and it has been identified as a novel type of Cd hyperaccumulator that can be harnessed for phytoremediation. However, the molecular mechanisms underlying the response of P. acinosa to Cd2+ stress remain largely unclear. In this study, the phenotype, biochemical, and physiological traits of P. acinosa seeds and seedlings were analyzed under different concentrations of Cd2+ treatments. The results showed higher Cd2+ tolerance of P. acinosa compared to common plants. Meanwhile, the Cd2+ content in shoots reached 449 mg/kg under 10 mg/L Cd2+ treatment, which was obviously higher than the threshold for Cd hyperaccumulators. To investigate the molecular mechanism underlying the adaptability of P. acinosa to Cd stress, RNA-Seq was used to examine transcriptional responses of P. acinosa to Cd stress. Transcriptome analysis found that 61 genes encoding TFs, 48 cell wall-related genes, 35 secondary metabolism-related genes, 133 membrane proteins and ion transporters, and 96 defense system-related genes were differentially expressed under Cd2+ stress, indicating that a series of genes were involved in Cd2+ stress, forming a complex signaling regulatory mechanism. These results provide new scientific evidence for elucidating the regulatory mechanisms of P. acinosa response to Cd2+ stress and new clues for the molecular breeding of heavy metal phytoremediation.
Funder
Scientific Research Fund of Hunan Provincial Education Department
Science and Education Joint Fund of Natural Science Foundation of Hunan Province
Natural Science Foundation of Changsha City
Foundation of Xiangnan University
Reference83 articles.
1. Slow release chelate enhancement of lead phytoextraction by corn (Zea mays L.) from contaminated soil-a preliminary study;Li;Sci. Total Environ.,2005
2. Leaf chlorosis in oilseed rape plants (Brassica napus) grown on cadmium-polluted soil: Causes and consequences for photosynthesis and growth;Baryla;Planta,2001
3. Synthesis and characterization of microbial mediated cadmium oxide nanopartciles;Asghar;Microsc. Res. Tech.,2020
4. Kaleem, M., Minhas, L.A., Hashmi, M.Z., Ali, M.A., Mahmoud, R.M., Saqib, S., Nazish, M., Zaman, W., and Mumtaz, A.S. (2023). Biosorption of Cadmium and Lead by Dry Biomass of Nostoc sp. MK-11 Kinetic and Isotherm Study. Molecules, 28.
5. Remediation technologies and current problems of heavy metal contaminated sites with ‘Demonstration project of soil remediation on the Periphery of Guixi Smelter’ as example;Zhou;Soil,2015
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献