Transcriptome Analysis for Salt-Responsive Genes in Two Different Alfalfa (Medicago sativa L.) Cultivars and Functional Analysis of MsHPCA1

Author:

Gao Qican1,Yu Ruonan12,Ma Xuesong1,Wuriyanghan Hada1,Yan Fang1

Affiliation:

1. Key Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China

2. Crop Cultivation and Genetic Improvement Research Center, College of Agricultural, Hulunbuir University, Hulunbuir 021008, China

Abstract

Alfalfa (Medicago sativa L.) is an important forage legume and soil salinization seriously affects its growth and yield. In a previous study, we identified a salt-tolerant variety ‘Gongnong NO.1’ and a salt-sensitive variety ‘Sibeide’. To unravel the molecular mechanism involved in salt stress, we conducted transcriptomic analysis on these two cultivars grown under 0 and 250 mM NaCl treatments for 0, 12, and 24 h. Totals of 336, and 548 differentially expressed genes (DEGs) in response to NaCl were, respectively, identified in the ‘Gongnong NO.1’ and ‘Sibeide’ varieties. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway enrichment analysis showed that the DEGs were classified in carbohydrate metabolism, energy production, transcription factor, and stress-associated pathway. Expression of MsHPCA1, encoding a putative H2O2 receptor, was responsive to both NaCl and H2O2 treatment. MsHPCA1 was localized in cell membrane and overexpression of MsHPCA1 in alfalfa increased salt tolerance and H2O2 content. This study will provide new gene resources for the improvement in salt tolerance in alfalfa and legume crops, which has important theoretical significance and potential application value.

Funder

Young Talents of Science and Technology

Innovative Research Team in Universities of Inner Mongolia Autonomous Region

Natural Science Foundation of Inner Mongolia Autonomous Region

Publisher

MDPI AG

Reference72 articles.

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