Integrated Metabolomics and Transcriptomics Analysis Reveals New Insights into Triterpene Biosynthesis in Rosa rugosa

Author:

Wei Guo1ORCID,Xu Yang1,Wang Pengqing1,Hussain Hammad1,Chen Yudie1,Shi Yuqing1,Zhu Kaikai2ORCID,Bai Mengjuan1,Xu Yong1,Wang Jianwen1ORCID,Feng Liguo1

Affiliation:

1. College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China

2. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Abstract

Rosa rugosa is highly regarded for its aesthetic and therapeutic qualities. In particular, R. rugosa’s flowers are known to produce essential oils containing a mixture of volatile terpenes, phenylpropanoids, and other compounds. Despite this, extensive research exists on volatile terpenes in flowers, while the knowledge of non-volatile terpenes in distinct tissues is still limited. Using UPLC–ESI–MS/MS, a comprehensive analysis of the terpene metabolites in five different tissues of R. rugosa was conducted. These metabolites accumulated in distinct tissues, and the majority of them were triterpenoids. Transcriptome data were collected from five tissues using RNA-seq. Transcriptomics and metabolomics were utilized to evaluate the triterpene biosynthesis pathway, resulting in new insights into its regulation and biosynthesis. The RrOSC10 was identified as a key enzyme in converting 2,3-oxidosqualene into α-amyrin, potentially contributing to the triterpene biosynthesis pathway. Furthermore, the expression of the RrOSC10 gene was upregulated by salinity for 0.5 h and 1 h, with subsequent downregulation at 2 h. This study lays a foundation for future research on the biosynthesis and accumulation of triterpenes in R. rugosa.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Postgraduate Research and Practice Innovation Program of Jiangsu Province

Publisher

MDPI AG

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