The Identification and Analysis of the Self-Incompatibility Pollen Determinant Factor SLF in Lycium barbarum

Author:

Wu Jiali1,Nan Xiongxiong2,Zhang Xin13,Xu Wendi13,Ma Haijun14,Yang Zijun1,Wang Cuiping13

Affiliation:

1. School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China

2. State Key Laboratory of Efficient Production of Forest Resources, Yinchuan 750004, China

3. Innovation Team for Genetic Improvement of Economic Forests, North Minzu University, Yinchuan 750021, China

4. Ningxia Grape and Wine Innovation Center, North Minzu University, Yinchuan 750021, China

Abstract

Self-incompatibility is a widespread genetic mechanism found in flowering plants. It plays a crucial role in preventing inbreeding and promoting outcrossing. The genes that control self-incompatibility in plants are typically determined by the S-locus female determinant factor and the S-locus male determinant factor. In the Solanaceae family, the male determinant factor is often the SLF gene. In this research, we cloned and analyzed 13 S2-LbSLF genes from the L. barbarum genome, which are located on chromosome 2 and close to the physical location of the S-locus female determinant factor S-RNase, covering a region of approximately 90.4 Mb. The amino acid sequence identity of the 13 S2-LbSLFs is 58.46%, and they all possess relatively conserved motifs and typical F-box domains, without introns. A co-linearity analysis revealed that there are no tandemly repeated genes in the S2-LbSLF genes, and that there are two pairs of co-linear genes between S2-LbSLF and the tomato, which also belongs to the Solanaceae family. A phylogenetic analysis indicates that the S2-LbSLF members can be divided into six groups, and it was found that the 13 S2-LbSLFs are clustered with the SLF genes of tobacco and Petunia inflata to varying degrees, potentially serving as pollen determinant factors regulating self-incompatibility in L. barbarum. The results for the gene expression patterns suggest that S2-LbSLF is only expressed in pollen tissue. The results of the yeast two-hybrid assay showed that the C-terminal region of S2-LbSLFs lacking the F-box domain can interact with S-RNase. This study provides theoretical data for further investigation into the functions of S2-LbSLF members, particularly for the identification of pollen determinant factors regulating self-incompatibility in L. barbarum.

Funder

Fundamental Research Funds for the Central Universities of North Minzu University

West Light Talent Program of the Chinese Academy of Sciences

National Natural Science Foundation of China

Innovation Team for Genetic Improvement of Economic Forests

Publisher

MDPI AG

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