In Vitro Propagation and Conservation of Lavandula stoechas subsp. luisieri and Pterospartum tridentatum, Two Important Medicinal and Aromatic Species from Portugal

Author:

Domingues Joana1,Eira Anabela1ORCID,Ramalho Isa1,Barrocas Inês1,Gonçalves José Carlos123ORCID

Affiliation:

1. Centro de Biotecnologia de Plantas da Beira Interior, Quinta Senhora de Mércules, 6000-191 Castelo Branco, Portugal

2. Polytechnic Institute of Castelo Branco-School of Agriculture (IPCB-ESA), Quinta Senhora de Mércules, 6000-191 Castelo Branco, Portugal

3. Research Center for Natural Resources, Environment and Society (CERNAS), Polytechnic Institute of Castelo Branco, Av. Pedro Álvares Cabral, 12, 6000-084 Castelo Branco, Portugal

Abstract

Lavandula stoechas subsp. luisieri and Pterospartum tridentatum are two valuable aromatic and medicinal plants. Their biometric and morphological parameters, such as the number of new shoots, length of the longest shoot, multiplication rate, and fresh weight, were evaluated using the multiplication MS medium protocol. The rooting protocols involved immersing the explants in IBA (1 g L−1) and a commercial IBA (3.3 g L−1) preparation (Clonex®). Slow-growth conservation assays were carried out using two different sucrose concentrations (15 g L−1 and 30 g L−1), and a control, with the cultures kept at 4 °C for 12 months. The multiplication rate for L. stoechas subsp. luisieri was 6.8, and that of P. tridentatum was 13.3, achieved using the MS medium supplemented with 0.2 mg L−1 BAP, 1 mg L−1 BAP, and 0.5 mg L−1 IBA. The application of Clonex® showed the best ex vitro rooting results in L. stoechas subsp. luisieri (77%) and P. tridentatum (90%). In the slow-growth conservation assays, at 4 °C, in darkness for 12 months, an excellent survival rate was achieved in L. stoechas subsp. luisieri (>80%) and P. tridentatum (>90%), even at the reduced sucrose concentration. This study demonstrates the effectiveness of in vitro multiplication and ex vitro rooting protocols for two valuable aromatic and medicinal plants. These findings are significant for the ex situ conservation of these species, as they provide effective long-term preservation and utilization strategies.

Publisher

MDPI AG

Reference37 articles.

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5. Effect of conservation on steroids contents of callus explants of date palm cv. sakkoti;Zaid;Aust. J. Basic Appl. Sci.,2012

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