Genetic Dissection of Diverse Seed Coat Patterns in Cowpea through a Comprehensive GWAS Approach-Reference-Cited by-同舟云学术

Genetic Dissection of Diverse Seed Coat Patterns in Cowpea through a Comprehensive GWAS Approach

Published:2024-05-05 Issue:9 Volume:13 Page:1275
ISSN:2223-7747
Container-title:Plants
language:en
Short-container-title:Plants

Author:

Xiong Haizheng¹,Chen Yilin¹^ORCID,Ravelombola Waltram²,Mou Beiquan³,Sun Xiaolun⁴^ORCID,Zhang Qingyang⁵,Xiao Yiting⁶,Tian Yang⁷^ORCID,Luo Qun¹,Alatawi Ibtisam¹,Chiwina Kenani Edward¹,Alkabkabi Hanan Mohammedsaeed¹,Shi Ainong¹^ORCID

Affiliation:

1. Department of Horticulture, University of Arkansas, Fayetteville, AR 72701, USA

2. Texas A&M AgriLife Research, 11708 Highway 70 South, Vernon, TX 76384, USA

3. Sam Farr U.S. Crop Improvement and Protection Research Center, U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS), Salinas, CA 93905, USA

4. Department of Poultry Science & The Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA

5. Mathematical Sciences, University of Arkansas, Fayetteville, AR 72701, USA

6. Biological Engineering, University of Arkansas, Fayetteville, AR 72701, USA

7. Program of Material Science and Engineering, Fayetteville, AR 72701, USA

Abstract

This study investigates the genetic determinants of seed coat color and pattern variations in cowpea (Vigna unguiculata), employing a genome-wide association approach. Analyzing a mapping panel of 296 cowpea varieties with 110,000 single nucleotide polymorphisms (SNPs), we focused on eight unique coat patterns: (1) Red and (2) Cream seed; (3) White and (4) Brown/Tan seed coat; (5) Pink, (6) Black, (7) Browneye and (8) Red/Brown Holstein. Across six GWAS models (GLM, SRM, MLM, MLMM, FarmCPU from GAPIT3, and TASSEL5), 13 significant SNP markers were identified and led to the discovery of 23 candidate genes. Among these, four specific genes may play a direct role in determining seed coat pigment. These findings lay a foundational basis for future breeding programs aimed at creating cowpea varieties aligned with consumer preferences and market requirements.

Funder

USDA Crop Germplasm Committees program for Vigna germplasm evaluation

USDA National Institute of Food and Agriculture Hatch project accession

Publisher

MDPI AG

Link

https://www.mdpi.com/2223-7747/13/9/1275/pdf

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