Olive Leaf Mottling Virus: A New Member of the Genus Olivavirus-Reference-Cited by-同舟云学术

Olive Leaf Mottling Virus: A New Member of the Genus Olivavirus

Published:2024-08-17 Issue:16 Volume:13 Page:2290
ISSN:2223-7747
Container-title:Plants
language:en
Short-container-title:Plants

Author:

Ruiz-García Ana Belén¹^ORCID,Candresse Thierry²^ORCID,Malagón José³,Ruiz-Torres Manuel⁴,Paz Sergio³,Pérez-Sierra Ana¹^ORCID,Olmos Antonio¹^ORCID

Affiliation:

1. Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra CV-315, km 10.7, 46113 Moncada, Valencia, Spain

2. UMR BFP, INRAE, University of Bordeaux, CS 20032, 33882 Villenave d’Ornon CEDEX, France

3. Servicio de Transferencia de Tecnología (STT) de la Generalitat Valenciana, Ctra CV-315, km 10.7, 46113 Moncada, Valencia, Spain

4. Laboratorio de Producción y Sanidad Vegetal de Jaén, Junta de Andalucía, Sierra Morena, 12b, 23620 Mengíbar, Jaén, Spain

Abstract

Studies of the virome of olive trees with symptoms of leaf mottling by high-throughput sequencing (HTS) revealed the presence of a new virus. Full coding genome sequences of two isolates were determined and consisted of a single RNA segment of 16,516 nt and 16,489, respectively. The genomic organization contained 10 open reading frames (ORFs) from 5′ to 3′: ORF1a, ORF1b (RdRp), ORF2 (p22), ORF3 (p7), ORF4 (HSP70h), ORF5 (HSP90h), ORF6 (CP), ORF7 (p19), ORF8 (p12), ORF9 (p23) and ORF10 (p9). Phylogenetic analyses clustered this virus in the genus Olivavirus, family Closteroviridae, with the closest species being Olivavirus flaviolae, commonly named olive leaf yellowing-associated virus (OLYaV). However, amino acid sequences of all taxonomically relevant proteins showed, in all cases, a divergence higher than 25% between OLYaV and the new virus, indicating that it represents a new species in the genus Olivavirus for which the common name of olive leaf mottling virus (OLMV) is proposed. This study represents an advance in the genus Olivavirus and provides new insights into the olive virome.

Funder

Agencia Estatal de Investigación

Publisher

MDPI AG

Link

https://www.mdpi.com/2223-7747/13/16/2290/pdf

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