Affiliation:
1. School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China
2. Henan Province Engineering Center of Horticulture Plant Resource Utilization and Germplasm Enhancement, Xinxiang 453003, China
Abstract
Kunitz trypsin inhibitor genes play important roles in stress resistance. In this study, we investigated RpKTI2 cloned from Robinia pseudoacacia and its effect on tobacco. RpKTI2 was introduced into the tobacco cultivar NC89 using Agrobacterium-mediated transformation. Six RpKTI2-overexpressing lines were obtained. Transgenic and wild-type tobacco plants were then compared for photosynthetic characteristics and endogenous hormone levels. Transgenic tobacco showed minor changes in chlorophyll content, fluorescence, and photosynthetic functions. However, the maximum photochemical efficiency (Fv/Fm) increased significantly while intercellular CO2 concentration (Ci) decreased significantly. Stomatal size and hormone content (indole-3-acetic acid, zeatin riboside, gibberellin, and indole-3-propionic acid) were reduced, while brassinosteroid content increased. Random forest regression revealed that RpKTI2 overexpression had the biggest impact on carotenoid content, initial fluorescence, Ci, stomatal area, and indole-3-acetic acid. Overall, RpKTI2 overexpression minimally affected chlorophyll synthesis and photosynthetic system characteristics but influenced stomatal development and likely enhanced the antioxidant capacity of tobacco. These findings provide a basis for future in-depth research on RpKTI2.
Funder
Henan Province Key Project of Higher Education Institution, China
Reference48 articles.
1. Lu, Y. (2016). Trypsin Inhibitor and Its Influence on Immunological Enhancement of Mulberry in Mice. [Master’s Thesis, Jiangsu University]. (In Chinese).
2. Research advances in Kunitz trypsin inhibitor;Luo;Chin. J. Biochem. Pharm.,2012
3. Chang, X. (2012). Insect Resistance Mechanisms of KTI Gene Families in Model Plants. [Master’s Thesis, Tianjin University]. (In Chinese).
4. Arnaiz, A., Talavera-Mateo, L., Gonzalez-Melendi, P., Martinez, M., Isabel, D., and Santamaria, M.E. (2018). Arabidopsis kunitz trypsin inhibitors in defense against spider mites. Front. Plant Sci., 9.
5. Sultana, M.S., Mazarei, M., Jurat-Fuentes, J.L., Hewezi, T., Millwood, R.J., and Stewart, C.N. (2023). Overexpression of soybean trypsin inhibitor genes decreases defoliation by corn earworm (Helicoverpa zea) in soybean (Glycine max) and Arabidopsis thaliana. Front. Plant Sci., 14.