Analysis of the NAC Gene Family in Salix and the Identification of SpsNAC005 Gene Contributing to Salt and Drought Tolerance

Abstract

The NAC gene family is of great value for plant stress resistance and development. In this study, five NAC genes with a typical NAM domain were isolated from Salix psammophila, which is a stress-resistant willow endemic to western China. Two hundred sixty-two NAC genes from Salix psammophila, Salix purpurea, and Arabidopsis were used to construct the phylogenetic tree to examine the phylogenetic relationship. Five NAC genes in Salix psammophila were the focus of bioinformatics analysis and conserved structural domain analysis. The SpsNAC005 gene was overexpressed in Populus hopeiensis, and the transgenic lines were subjected to salt and simulated drought stress to analyze their phenotype changes and tolerance to stress. The results showed that transgenic poplar height and leaf area increased by 29.73% and 76.36%, respectively, compared with those of wild-type plants. Under stress treatment, the height growth rates and ground diameter growth rates of the transgenic lines were significantly higher than those of the wild-type, whereas their fresh weight and dry weight were decreased compared to those of the wild-type. The SOD activities, POD activities, and Pro contents of the transgenic plants were significantly increased, and the accumulation of MDA was significantly lower than that in the wild-type, and the transgenic lines showed clear tolerance to salt and drought. The expressions of the SOS1, MPK6, HKT1, and P5CS1 genes were downregulated in the transgenic lines. The expression of the PRODH1 gene was downregulated in the transgenic lines. These results indicate that overexpression of the SpsNAC005 gene in transgenic plants can promote plant growth and development and improve tolerance to salt and drought.