Post-Orthodontic Relapse Prevention through Administration of a Novel Synthetic Carbonated Hydroxyapatite–Chitosan Hydrogel Derived from Blood Cockle Shell (Anadara granosa L.)
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Published:2024-01-19
Issue:1
Volume:12
Page:18
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ISSN:2304-6767
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Container-title:Dentistry Journal
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language:en
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Short-container-title:Dentistry Journal
Author:
Hadi Aanisah Fauziyyah Nurul1, Aghniya Sabrina Noor1, Haidar Gayuh Abi1, Sihombing Windy Sepry Marcelina1, Sutedjo Angelina1, Alhasyimi Ananto Ali2ORCID
Affiliation:
1. Undergraduate Program, Faculty of Dentistry, Gadjah Mada University, Sleman, Yogyakarta 55281, Indonesia 2. Department of Orthodontic, Faculty of Dentistry, Gadjah Mada University, Sleman, Yogyakarta 55281, Indonesia
Abstract
Relapse during passive orthodontic treatment is a major issue, with 70–90% frequency. This study examines whether blood cockle shells may be used to extract carbonated hydroxyapatite (CHA)-chitosan (CS). This study also aims to analyze the effect of CHA-CS on orthodontic relapse in rats. This study utilized 18 male Wistar rats which were randomly divided into two groups: CHA-CS and the control group (CG). The rats were subjected to a 35 cN orthodontic force for a duration of 7 days, after which the rats were conditioned to be passive. During this phase, the CHA-CS group received daily administration of CHA-CS hydrogel derived from the blood cockle shell. Subsequently, the appliances were detached to facilitate relapse. The distance between the mesial tips was measured using a digital caliper at three consecutive time points: 1, 5, and 7 days after debonding. The number of osteoblasts, osteoclasts, and fibroblasts was examined using hematoxylin–eosin staining. The data were subjected to statistical analysis using a t-test. The relapse distance of the CHA-CS group was lower than that of the control groups on day 7. Histological examinations using hematoxylin–eosin (HE) staining showed a significant increase in osteoblasts, a decrease in osteoclasts, and an increase in fibroblasts during orthodontic relapse movement (p < 0.05). This study found that blood cockle shell-derived CHA-CS may reduce orthodontic relapse by increasing osteoblasts and fibroblasts and by reducing the osteoclast number in rats.
Funder
Student Creativity Program
Subject
General Dentistry
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