Functional and Quality Assessment of a Spore Harvester for Entomopathogenic Fungi for Biopesticide Production
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Published:2023-04-28
Issue:2
Volume:5
Page:801-813
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ISSN:2624-7402
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Container-title:AgriEngineering
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language:en
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Short-container-title:AgriEngineering
Author:
Diego-Nava Fidel1, Granados-Echegoyen Carlos2, Ruíz-Vega Jaime1, Aquino-Bolaños Teodulfo1, Pérez-Pacheco Rafael1, Díaz-Ramos Alejo1, Alonso-Hernández Nancy1, Arroyo-Balán Fabián2ORCID, López-Hernández Mónica Beatriz3
Affiliation:
1. CIIDIR Unidad Oaxaca, Instituto Politécnico Nacional, Santa Cruz Xoxocotlán 71230, Mexico 2. Laboratorio de Entomología Aplicada, Centro de Estudios en Desarrollo Sustentable y Aprovechamiento de la Vida Silvestre (CEDESU), CONACYT-Universidad Autónoma de Campeche, San Francisco de Campeche 24079, Mexico 3. Instituto Tecnológico de Chiná (ITChiná), Tecnológico Nacional de Mexico, Chiná 24520, Mexico
Abstract
The Green Revolution led to an increased use of synthetic pesticides, causing environmental pollution. As an alternative, biopesticides made from entomopathogenic agents such as fungi have been sought. This study aimed to design and evaluate the performance of a harvester machine for efficiently collecting entomopathogenic spores of Metarhizium anisopliae and Beauveria bassiana grown on rice and corn substrates. The spore yield was estimated, and a spore count and a colony-forming unit (CFU) count were performed. Statistical analysis was conducted to compare the mean values of spores obtained from different combinations of solid substrate and fungi. The Corn-Metarhizium combination produced 34.15 g of spores per kg of substrate and 1.51 × 109 CFUs mL−1. Similarly, the Rice-Metarhizium combination produced 57.35 g per kg and 1.59 × 109 CFUs mL−1. Meanwhile, the Corn-Beauveria combination yielded 35.47 g per kg and 1.00 × 109 CFUs mL−1, while the Rice-Beauveria combination had a yield of 38.26 g per kg and 4.50 × 108 CFUs mL−1. Based on the reported results, the Rice-Metarhizium combination appears to be the most effective, yielding the highest number of harvested spores per kg of substrate. The study estimated a total cost of approximately $409.31 for manufacturing the harvester, considering only the cost of the materials. These results could potentially increase the availability and affordability of entomopathogenic fungi in integrated pest management.
Subject
Engineering (miscellaneous),Horticulture,Food Science,Agronomy and Crop Science
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