Effects of Upwelling Intensity on Nitrogen and Carbon Fluxes through the Planktonic Food Web off A Coruña (Galicia, NW Spain) Assessed with Stable Isotopes

Abstract

The input of new nutrients by upwelling in shelf waters, and the associated carbon and nitrogen fluxes, can be traced by their stable isotope signatures in organic matter. Here, we analyze the relationships between upwelling intensity and natural abundance of stable carbon and nitrogen isotopes in seston, sedimented particles, and four plankton size fractions (200 to 5000 µm) sampled monthly during 2010 and 2011 in a seasonal upwelling ecosystem. Upwelling modified the seasonal warming stratification by introducing cold and nutrient-rich waters in subsurface layers, enhancing chlorophyll-a and diatom abundance. Seston and sedimented particles were enriched in heavy nitrogen (but not carbon) isotopes linearly with upwelling intensity, indicating a primary effect of upwelling on phytoplankton production. In contrast, all plankton fractions were enriched in heavy carbon isotopes with upwelling, mainly due to the consumption of diatoms. These results confirm the differential effect of upwelling on nitrogen and carbon fluxes in the plankton food web. Direct effects of the new nitrogen inputs on phytoplankton are less evident with the increase of plankton size as nitrogen is repeatedly recycled, while the enriched carbon of plankton suggests the consumption of diatoms during upwelling. We provide linear equations to assess the influence of changes in upwelling intensity on nitrogen and carbon fluxes in seston and plankton in this ecosystem, as well as to estimate reference baseline values for food web studies.