Affiliation:
1. College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
2. Faculty of Health Sciences, University of Macau, Macau SAR, China
3. Guangzhou Institute of Modern Industrial Technology, Guangzhou 511458, China
Abstract
In this study, experimental tests, together with computer analyses, were carried out to identify the essential nutritional markers that can offer protein supplementation-based nutrition support for hypertension. In particular, 16 marine shellfish proteins were firstly screened for in silico hydrolysis by gastrointestinal enzymes and then the binding abilities of the obtained oligopeptides to angiotensin-converting enzyme (ACE) were examined. In addition, the ACE inhibition activities of selected oligopeptides were assayed in vitro. It was discovered that mussel-derived NADH dehydrogenase and AMP-activated protein kinase and razor-clam-derived cytochrome b, NADH dehydrogenase, and ATP synthase were excellent sources of protein that are able to release a high amount of ACE-binding peptides. Meanwhile, four oligopeptides (SCCGY, SSGAW, PICQF, and IQPEW) were detected as strong ACE binders, with IC50 values of 0.09, 0.25, 0.41, and 0.60 mM, respectively. Therefore, our findings demonstrate that shellfish proteins can serve as nutritional support for hypertension when patients are supplemented with these markers. In particular, oligopeptides are identified as the most promising compounds.
Funder
The Program of The Department of Natural Resources of Guangdong Province
Subject
Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science
Cited by
1 articles.
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