Resveratrol Ameliorates Testicular Histopathology of Mice Exposed to Restraint Stress

Author:

Mustafa SheerazORCID,Wei Quanwei,Ennab Wael,Lv Zengpeng,Nazar Korejo,Siyal Farman,Rodeni Saif,Kavita Ngekure,Shi FangxiongORCID

Abstract

We evaluated immobilization stress and resveratrol supplementation in immature male mice at 30 days of age for 15 consecutive days. Fifty Swiss mice were divided into five groups (10 mice each): Controls, restraint stress (RS), restraint stress + vehicle (RS + V), RS + 2 mg/kg, and RS + 20 mg/kg. We determined results on the basis of hematoxylin and eosin (H&E), “Periodic acid-Schiff” staining, and TUNEL assay. The results indicated that immobilization stress significantly decreased body weight, testis weight, and water/food intake compared to the control; while resveratrol ameliorated these effects. The quantitative histologic evaluation of the seminiferous tubule diameter, luminal diameter, area of seminiferous tubules, area of tubule lumen, epithelial height, Leydig cell number, and the width of the tunica albuginea were similarly decreased after exposure to RS. These parameters recovered back to normal in the RS + 2 mg/kg group. The development of spermatogenesis was significantly delayed in the RS, RS + V, and RS + 20 mg groups based upon our evaluation score system. However, we observed no significant differences in the RS + 2 mg group compared with the control group. The number of TUNEL-positive cells also significantly decreased in the RS + 2 mg/kg group. In conclusion, we found that the administration of 2 mg/kg was an effective dose against immobilization stress in mice.

Funder

The Chinese National Foundation for Natural Science

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

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