Pulsed Radiofrequency Electromagnetic Fields as Modulators of Inflammation and Wound Healing in Primary Dermal Fibroblasts of Ulcers

Author:

Costantini Erica1ORCID,Aielli Lisa2ORCID,Gualdi Giulio1ORCID,Baronio Manuela3,Monari Paola4,Amerio Paolo1ORCID,Reale Marcella2ORCID

Affiliation:

1. Department of Medicine and Aging Sciences, University “G. d’Annunzio”, 66100 Chieti, Italy

2. Department of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio”, 66100 Chieti, Italy

3. Pediatrics Clinic and Institute for Molecular Medicine A. Novivelli, Department of Clinical and Expermental Sciences, University of Brescia and ASST-Spedali Civili of Brescia, 25123 Brescia, Italy

4. Department of Dermatology, Spedali Civili of Brescia, 25123 Brescia, Italy

Abstract

Venous leg ulcers are one of the most common nonhealing conditions and represent an important clinical problem. The application of pulsed radiofrequency electromagnetic fields (PRF-EMFs), already applied for pain, inflammation, and new tissue formation, can represent a promising approach for venous leg ulcer amelioration. This study aims to evaluate the effect of PRF-EMF exposure on the inflammatory, antioxidant, cell proliferation, and wound healing characteristics of human primary dermal fibroblasts collected from venous leg ulcer patients. The cells’ proliferative and migratory abilities were evaluated by means of a BrdU assay and scratch assay, respectively. The inflammatory response was investigated through TNFα, TGFβ, COX2, IL6, and IL1β gene expression analysis and PGE2 and IL1β production, while the antioxidant activity was tested by measuring GSH, GSSG, tGSH, and GR levels. This study emphasizes the ability of PRF-EMFs to modulate the TGFβ, COX2, IL6, IL1β, and TNFα gene expression in exposed ulcers. Moreover, it confirms the improvement of the proliferative index and wound healing ability presented by PRF-EMFs. In conclusion, exposure to PRF-EMFs can represent a strategy to help tissue repair, regulating mediators involved in the wound healing process.

Publisher

MDPI AG

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