Non-Invasive Monitoring of Cutaneous Wound Healing in Non-Diabetic and Diabetic Model of Adult Zebrafish Using OCT Angiography

Author:

Kim Jaeyoung123ORCID,Kim Suhyun45ORCID,Choi Woo June6ORCID

Affiliation:

1. Research Institute for Skin Image, Korea University College of Medicine, Seoul 08308, Republic of Korea

2. Department of Dermatology and Skin Science, University of British Columbia, Vancouver, BC V6T 1Z1, Canada

3. Departments of Cancer Control Research and Integrative Oncology, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada

4. Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Republic of Korea

5. Zebrafish Translational Medical Research Center, Korea University Ansan Hospital, Ansan 15355, Republic of Korea

6. School of Electrical and Electronics Engineering, Chung-Ang University, Seoul 06974, Republic of Korea

Abstract

A diabetic wound presents a severe risk of infections and other complications because of its slow healing. Evaluating the pathophysiology during wound healing is imperative for wound care, necessitating a proper diabetic wound model and assay for monitoring. The adult zebrafish is a rapid and robust model for studying human cutaneous wound healing because of its fecundity and high similarities to human wound repair. OCTA as an assay can provide three-dimensional (3D) imaging of the tissue structure and vasculature in the epidermis, enabling monitoring of the pathophysiologic alterations in the zebrafish skin wound. We present a longitudinal study for assessing the cutaneous wound healing of the diabetic adult zebrafish model using OCTA, which is of importance for the diabetes research using the alternative animal models. We used non-diabetic (n = 9) and type 1 diabetes mellitus (DM) adult zebrafish models (n = 9). The full-thickness wound was generated on the fish skin, and the wound healing was monitored with OCTA for 15 days. The OCTA results demonstrated significant differences between diabetic and non-diabetic wound healing, involving delayed tissue remodeling and impaired angiogenesis for the diabetic wound, leading to slow wound recovery. The adult zebrafish model and OCTA technique may benefit long-term metabolic disease studies using zebrafish for drug development.

Funder

Ministry of Education

Korean government

Technology Innovation Program

Publisher

MDPI AG

Subject

Bioengineering

Reference65 articles.

1. American Diabetes Association (2010). Diagnosis and Classification of Diabetes Mellitus. Diabetes Care, 33, S62–S69.

2. International Diabetes Federation (2022, November 29). IDF Diabetes Atlas 2021: IDF Diabetes Atlas 10th Edition. Available online: https://diabetesatlas.org/atlas/tenth-edition/.

3. Mechanism of diabetic neuropathy: Where are we now and where to go?;Yagihashi;J. Diabetes Investig.,2011

4. Weledji, E.P., and Fokam, P. (2014). Treatment of the diabetic foot–to amputate or not?. BMC Surg., 14.

5. Cutaneous Wound Healing;Singer;N. Engl. J. Med.,1999

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3