Abstract
Axial suspension plasma spraying (ASPS) is an alternative technique to atmospheric plasma spraying (APS), which uses a suspension of much finer powders (<5-micron particle size) as the feedstock. It can produce more refined microstructures than APS for biomedical implants. This paper highlights the influence of incorporated graphene nanoplatelets (GNPs) on the behavior of ASPS hydroxyapatite (HAp) coatings. The characterization of the ASPS coatings (HAp + varying GNP contents) was carried out using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), confocal Raman microscopy (CRM), white light interferometry (WLI), and contact angle measurements. The evaluation of the mechanical properties such as the hardness, roughness, adhesion strength, and porosity was carried out, along with a fretting wear performance. Additionally, the biocompatibility of the Hap + GNP coatings was evaluated using cytotoxicity testing which revealed a decrease in the cell viability from 92.7% to 85.4%, with an increase in the GNP wt.%. The visualization of the cell’s components was carried out using SEM and Laser Scanning Microscopy. Furthermore, the changes in the genetic expression of the various cellular markers were assessed to analyze the epigenetic changes in human mesenchymal stem cells. The gene expression changes suggested that GNPs upregulated the proliferation marker and downregulated the pluripotent markers by a minimum of three folds.
Funder
Department of Science and Technology
Swedish Research Council
Reference34 articles.
1. (2022, February 28). Guidance for Industry on the Testing of Metallic Plasma Sprayed Coatings on Orthopedic Implants to Support Reconsideration of Postmarket Surveillance Requirements—Guidance for Industry and for FDA Reviewers/Staff|FDA, Available online: https://www.fda.gov/regulatory-information/search-fda-guidance-documents/guidance-industry-testing-metallic-plasma-sprayed-coatings-orthopedic-implants-support.
2. The History, Technical Specifications and Efficacy of Plasma Spray Coatings Applied to Joint Replacement Prostheses;McCabe;Reconstr. Rev.,2016
3. Axial Suspension Plasma Spraying: An Ultimate Technique to Tailor Ti6Al4V Surface with HAp for Orthopaedic Applications;Hameed;Colloids Surf. B Biointerfaces,2019
4. Plasma-Sprayed Hydroxylapatite Coatings as Biocompatible Intermediaries Between Inorganic Implant Surfaces and Living Tissue;Heimann;J. Therm. Spray Technol.,2018
5. Deposition of Hydroxyapatite Coatings by Axial Plasma Spraying: Influence of Feedstock Characteristics on Coating Microstructure, Phase Content and Mechanical Properties;Ganvir;J. Eur. Ceram. Soc.,2021
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献