Effective Treatments for the Successful Establishment of Milkweed (Calotropis procera L.) under Water Deficit

Abstract

The application of superabsorbents to soils and seed coatings is a pre-sowing seed treatment method that is commonly used to improve early vigor and establish stability and uniformity under water deficit conditions. To evaluate the interaction of seed coating and superabsorbent on Calotropisprocera L. (milkweed) under water deficit conditions, a greenhouse experiment was conducted. The experiment was conducted with four coating material levels (non-coated seeds and seeds coated with peat moss, vermicompost, and canola residue), four growth medium levels (soil, sand + soil, soil + 2 g superabsorbent, and soil + 4 g superabsorbent), and three field capacity regimes (25, 50%, and 100%) in a completely randomized design factorial arrangement with four replications. Reducing the field capacity from 100 to 25% led to decreased growth (shoot and root dry weights and leaf area) and chlorophyll content. The activities of SOD, CAT, APX antioxidant enzymes, and proline increased under drought stress. The use of superabsorbent polymers in growth media enhanced growth indices and chlorophyll content and decreased the activity of antioxidant enzymes and proline under water deficit conditions. The highest chlorophyll and growth indices were observed when 4 g of superabsorbent was added to the growth medium under drought stress. The application of 4 g of superabsorbent to the growth medium reduced the activity of antioxidant enzymes and proline. The use of seed coatings improved the growth indices, antioxidant enzyme activity, and chlorophyll content under drought stress. The most adaptive morphological and physiological responses to water stress were observed in the vermicompost-coated seeds. The vermicompost coating containing a superabsorbent polymer (4 g/kg soil) proved to be the best for establishing milkweed under mild (50% FC) and severe water deficits (25% FC).