The Magic Cocktail: Ampicillin and Biosynthesized Gold Nanoparticles Synergism against Staphylococcus aureus

Author:

Oliveira Marco123,Soares Silvia145ORCID,Sá Sara145ORCID,Gestoso Álvaro14,Correa-Duarte Miguel67ORCID,Baylina Pilar148ORCID,Fernandes Rúben149ORCID,Pereira Carla F.3

Affiliation:

1. FP-BHS—Biomedical and Health Sciences Research Unit, FP-I3ID—Instituto de Investigação, Inovação e Desenvolvimento, FFP—Fundação Fernando Pessoa, 4420-096 Porto, Portugal

2. CTBIO—Polytechnic, Institute of Porto, 4420-096 Porto, Portugal

3. CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal

4. HE-FP, Hospital Fernando Pessoa, CECLIN—Center of Clinical Studies, 4420-096 Gondomar, Portugal

5. Faculty of Medicine, University of Porto, 4420-319 Porto, Portugal

6. CINBIO—Center for Research in Nanomaterials and Biomedicine, University of Vigo, 36310 Vigo, Spain

7. Faculty of Chemistry, Department of Physical Chemistry, University of Vigo, 36310 Vigo, Spain

8. School of Health of the Polytechnic, Institute of Porto, 4420-072 Porto, Portugal

9. Faculty of Health Sciences, Fernando Pessoa University, 420-150 Porto, Portugal

Abstract

Gold nanoparticles (AuNPs) have garnered attention as a potential alternative to conventional antibiotics due to their innovative antibacterial properties. This study demonstrates the successful production of biosynthetic gold nanoparticles (bAuNPs) using Pseudomonas aeruginosa (P. aeruginosa) as spherical nanostructures at 58 °C for 24 h, under alkaline pH (9.0). The successful synthesis of bAuNPs was confirmed through UV-Vis spectroscopy, exhibiting a characteristic peak within the 500–600 nm wavelength range, and the evaluation of the main functional groups and morphology were stressed by Fourier Transform Infrared Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM), respectively. Subsequently, the synthesized bAuNPs were combined with low concentrations of ampicillin and evaluated against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) through the classical serial dilution method. This innovative approach holds the potential to address the escalating issue of antibiotic resistance, providing a viable and sustainable solution.

Publisher

MDPI AG

Subject

Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science

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