Author:
Li ,Li ,Otsuka ,Zhang ,Takahashi ,Yamada
Abstract
We developed an easily accessible boron-dipyrromethene (BODIPY)-based fluorogenic probe, which we named LD-TB. This probe emits bright fluorescence in oil; when compared with aqueous solution, a significant enhancement of fluorescence brightness is observed. Cellular experiments confirmed that the probe stains the lipid droplets (LDs) specifically in both live and fixed cells, providing background-free images. Compared with Nile Red dye, a commonly used LD marker, LD-TB showed superior photostability. The sharp absorption and emission bands enable its multicolor imaging with blue and green probes. Importantly, the probe has proved to have low toxicity and is compatible with cell fixation. Our research provides a promising new fluorogenic probe for specific imaging of LDs.
Subject
General Materials Science
Reference34 articles.
1. The biophysics and cell biology of lipid droplets;Thiam;Nat. Rev. Mol. Cell Biol.,2013
2. The biogenesis and functions of lipid bodies in animals, plants and microorganisms;Murphy;Prog. Lipid Res.,2001
3. Lipid droplets finally get a little respect;Farese;Cell,2009
4. Opinion: Lipid droplets: A unified view of a dynamic organelle;Martin;Nat. Rev. Mol. Cell Biol.,2006
5. Lipid droplet-organelle interactions; sharing the fats;Murphy;Biochim. Biophys. Acta BBA Mol. Cell Biol. Lipids,2009
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