Enzymatic Formation of Recombinant Antibody-Conjugated Gold Nanoparticles in the Presence of Citrate Groups and Bacteria

Author:

Rad MaryamORCID,Ebrahimipour Gholamhossein,Bandehpour MojganORCID,Akhavan OmidORCID,Yarian Fatemeh

Abstract

With the spread of deadly diseases worldwide, the design of rapid tests to identify causative microorganisms is necessary. Due to the unique properties of gold nanoparticles, these nanoparticles are used in designing rapid diagnostic tests, such as strip tests. The current study aimed to investigate the ability of gold nanoparticles to bind to single-chain variable fragment antibodies. In this study, the biological and chemical methods included Escherichia coli TOP-10 and the Turkevich method to synthesize the gold nanoparticles, respectively. Then, the effect of synthetic nanoparticles on their capability of binding to recombinant antibodies was assessed by agarose gel and UV-vis spectroscopy. Our result showed that gold nanoparticles had a spherical morphology, and their average size was ~45 nm. Additionally, the citrate groups in gold nanoparticles were able to bind to serine residues in the antibody linker sequence; so, the chemical synthesis of gold nanoparticles is an effective strategy for binding these nanoparticles to antibodies that can be used in designing rapid diagnostic tests to promptly identify infectious microorganisms.

Publisher

MDPI AG

Subject

Physical and Theoretical Chemistry,Catalysis,General Environmental Science

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