Effect of Soil Microbiome from Church Forest in the Northwest Ethiopian Highlands on the Growth of Olea europaea and Albizia gummifera Seedlings under Glasshouse Conditions

Abstract

Loss of beneficial microbes and lack of native inoculum have hindered reforestation efforts in the severely-degraded lands worldwide. This is a particularly pressing problem for Ethiopia owing to centuries-old unsustainable agricultural practices. This study aimed to evaluate the inoculum potential of soils from church forest in the northwest highlands of Ethiopia and its effect on seedling growth of two selected native tree species (Olea europaea and Albizia gummifera) under a glasshouse environment. Seedlings germinated in a seed chamber were transplanted into pots containing sterilized and/or non-sterilized soils collected from under the canopy of three dominant church forest trees: Albizia gummifera (AG), Croton macrostachyus (CM), and Juniperus procera (JP) as well as from adjacent degraded land (DL). A total of 128 pots (2 plant species × 4 soil origins × 2 soil treatments × 8 replicates) were arranged in a factorial design. Overall, seedlings grown in AG, CM, and JP soils showed a higher plant performance and survival rate, as a result of higher soil microbial abundance and diversity, than those grown in DL soils. The results showed significantly higher plant height, root collar diameter, shoot, and total mass for seedlings grown in non-sterilized forest soils than those grown in sterilized soils. Furthermore, the bacterial relative abundance of Acidobacteria, Actinobacteria, and Nitrospirae was significantly higher in the non-sterilized forest soils AG, CM, and JP (r2 = 0.6–0.8, p < 0.001). Soil pH had a strong effect on abundance of the bacterial community in the church forest soils. More specifically, this study further demonstrated that the effect of soil microbiome was noticeable on the performance of Olea seedlings grown in the soil from CM. This suggests that the soils from remnant church forests, particularly from the canopy under CM, can serve as a good soil origin, which possibly would promote the native tree seedling growth and survival in degraded lands.