Y-Box-Binding Proteins Have a Dual Impact on Cellular Translation
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Published:2024-02-01
Issue:3
Volume:25
Page:1736
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Eliseeva Irina A.1, Buyan Andrey I.1ORCID, Smolin Egor A.1, Kaliadzenka Karina S.1, Popov Sergey2, Kulakovskiy Ivan V.1ORCID, Lyabin Dmitry N.1
Affiliation:
1. Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia 2. Endocrinology Research Center, Moscow 117036, Russia
Abstract
Y-box-binding proteins (YB proteins) are multifunctional DNA- and RNA-binding proteins that play an important role in the regulation of gene expression. The high homology of their cold shock domains and the similarity between their long, unstructured C-terminal domains suggest that Y-box-binding proteins may have similar functions in a cell. Here, we consider the functional interchangeability of the somatic YB proteins YB-1 and YB-3. RNA-seq and Ribo-seq are used to track changes in the mRNA abundance or mRNA translation in HEK293T cells solely expressing YB-1, YB-3, or neither of them. We show that YB proteins have a dual effect on translation. Although the expression of YB proteins stimulates global translation, YB-1 and YB-3 inhibit the translation of their direct CLIP-identified mRNA targets. The impact of YB-1 and YB-3 on the translation of their mRNA targets is similar, which suggests that they can substitute each other in inhibiting the translation of their mRNA targets in HEK293T cells.
Funder
RSF Ministry of Science and Higher Education of the Russian Federation
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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