Regulation of the Degradation Properties of Tyrosinase-Catalyzed Crosslinking Silk Membranes for Superficial Wound Repair
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Published:2024-06-11
Issue:12
Volume:17
Page:2839
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ISSN:1996-1944
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Container-title:Materials
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language:en
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Short-container-title:Materials
Author:
Liu Yu1ORCID, Liu Xuping1, Jiao Yuhong1, Li Mingzhong1ORCID
Affiliation:
1. National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China
Abstract
Appropriate biodegradability to meet the demands of wound repair is critical for superficial wound repair membrane applications. Tyrosinase-catalyzed crosslinking SF (c-SF) membranes were constructed and regulated the degradation behavior in this study. The crosslinking degree of the c-SF membranes could be adjusted by reaction ratios of tyrosinase against SF (TYR/SF). Upon reaching a TYR/SF ratio of 20/6000, the degree of crosslinking increased to 88.17 ± 0.20%, without obvious changes in the crystal structure. The degradation behavior was regulated by the TYR/SF ratio and the degradation environment. All c-SF membranes remained stable after immersion without collagenase but showed an adjustable degradation behavior in the presence of collagenase. As the TYR/SF ratio increased, the residual weights increased from 23.31 ± 1.35% to 60.12 ± 0.82% after 7 days of degradation, occurring with low increased amounts of β-sheet structure and free amino acids. This work provides a new c-SF membrane with controllable rapid degradability and favorable cytocompatibility, which can help to meet requirements for biodegradable superficial wound repair membranes.
Funder
Large-scale Scientific Instruments Open and Shared Independent Research Project of Jiangsu Province National Natural Science Foundation of China Natural Science Foundation of Jiangsu Province
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