Flowering Phenology and Characteristics of Pollen Aeroparticles of Quercus Species in Korea

Abstract

In recent decades, airborne allergens for allergic respiratory diseases have been found to increase significantly by a process of converting coniferous forests into broad-leaved forests in Korea. This study was conducted to evaluate factors, including airborne pollen counts, micromorphology, and flowering phenology, that can affect oak pollen-related allergic symptoms. The catkin of Mongolian oak (Quercus mongolica Fisch. ex Ledeb.) showed the most rapidly blooming catkin on Julian day 104 in flower development. Among six species, the last flowering was observed on Julian day 119 in Korean oak (Quercus dentata Thunb.). The pollen dispersal was persisted for about 32 days from Julian day 104 to Julian day 136. Airborne pollen was observed about 2 weeks after flowering phase H, the senescence phase. Pollen size varied by species, with the largest from Q. mongolica (polar axis length, PL = 31.72 µm, equatorial axis length, EL = 39.05 µm) and the smallest from Jolcham oak (Quercus serrata Murray) (PL = 26.47 µm, EL = 32.32 µm). Regarding pollen wall structure, endexine of Q. dentata was coarsely laminated or fragmented. The endexine thicknesses of Sawtooth oak (Quercus acutissima Carruth.) and Q. serrata were thick and stable, whereas Galcham oak (Quercus aliena Blume), Q. mongolica, and Oriental cork oak (Quercus variabilis Blume) had thinner endexines. The area occupied by pollenkitt of Q. variabilis was significantly larger than that of Q. acutissima. Importantly, Q. variabilis had a distinctly thick 17 kDa protein band, a presumed major allergen. Oak species differ in pollen protein composition, and thus there is a possibility that the allergenic activity of pollen proteins vary depending on oak species. This study highlights the fact that native oak species in Korea differ in flowering pattern of male flowers, pollen morphology, and pollen chemical constituents. These discrepancies in flowering and pollen properties imply variable allergic responses to oak pollen from different species.