Effects of Breeding Forest Musk Deer on Soil Bacterial Community Structure

Abstract

Breeding captive forest musk deer is an important way to obtain musk resources, but it also causes changes in the ecological environment of the breeding site. This study compared the changes in soil bacterial communities inside and outside the captive breeding site and tried to find out its influencing factors. High-throughput sequencing analysis and other methods were used to analyze the changes in the bacterial community structure in the natural soil and forest surrounding the musk deer site after 4 years of captivity. The results showed that the main dominant phyla in the captive soil samples were Proteobacteria, Acidobacteria and Chloroflexi. In order, Sphingomonadales (8.7%), Acidobacteriales (9.9%) and Solibacterales (6.1%) were dominant in the captive soil, while Rhizobiales (11.3%) and Gaiellales (6.2%) were found in non-captive soil. The main soil-specific microorganisms under captive conditions were Burkholderiales, Pseudomonadales and Sphingomadales. These microorganisms mainly gather at the order level and can be used as indicator microorganisms. A canonical correspondence analysis (CCA) showed that the microbial diversity in captivity soil was significantly affected by the contents of organic matter, available phosphorus and total nitrogen. These results will provide a basis for the healthy breeding of animals and shed light on the protection of the ecological environment in the Bashan Mountains of Qinling.