Affiliation:
1. Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland
2. Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland
3. Department of Forensic Medicine, Collegium Medicum, University of Zielona Góra, Zyty 28, 65-046 Zielona Góra, Poland
Abstract
The study aimed to explore in vivo the influence of cannabidiol (CBD) on the development of alcohol tolerance in rats. Rats were treated with ethanol (3.0 g/kg, i.p.) and CBD (20 mg/kg, p.o.) for nine successive days, and rectal body temperature, sedation (sleeping time), and blood alcohol concentration (BAC) were measured. In the prefrontal cortex, hippocampus, and striatum, the cannabinoid (CB1R and CB2R) and dopaminergic (DRD1, DRD2, DRD4, DRD5) receptors’ mRNA level changes were analyzed using the quantitative RT-PCR method. CBD inhibited the development of tolerance to the hypothermic and sedative action of alcohol, coupled with BAC elevation. On a molecular level, the most pronounced effects of the CBD + ethanol interaction in the striatum were observed, where CBD reversed the downregulation of CB2R gene transcription caused by ethanol. For CB1R, DRD1, and DRD2 mRNAs, the CBD + ethanol interaction produced opposite effects than for CB2R ones. In turn, for the transcription of genes encoding dopaminergic receptors, the most potent effect of alcohol as CBD occurred in the hippocampus. However, the combined CBD and alcohol administration showed the same effect for each substance administered separately. Since tolerance is considered a prelude to drug addiction, obtained results allow us to emphasize the thesis that CBD can inhibit the development of alcohol dependence in rats.
Funder
National Science Centre in Poland
Subject
Food Science,Nutrition and Dietetics
Reference95 articles.
1. State-of-the-art behavioral and pharmacological treatments for alcohol use disorder;Ray;Am. J. Drug Alcohol Abus.,2019
2. Does tolerance develop to the activating, as well as the depressant, effects of ethanol?;Tabakoff;Pharmacol. Biochem. Behav.,1982
3. Similar withdrawal severity in adolescents and adults in a rat model of alcohol dependence;Morris;Alcohol,2010
4. Szulc, M., Kujawski, R., Baraniak, J., Kania-Dobrowolska, M., Kamińska, E., Gryszczyńska, A., Czora-Poczwardowska, K., Winiarska, H., and Mikołajczak, P.Ł. (2021). Differential Influence of Pueraria lobata Root Extract and Its Main Isoflavones on Ghrelin Levels in Alcohol-Treated Rats. Pharmaceuticals, 15.
5. Acamprosate effect on short-term memory in chronically EtOH treated rats;Szulc;Pol. J. Pharmacol.,2002
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