Computational Biology and Machine Learning Approaches Identify Rubber Tree (Hevea brasiliensis Muell. Arg.) Genome Encoded MicroRNAs Targeting Rubber Tree Virus 1
-
Published:2022-12-15
Issue:24
Volume:12
Page:12908
-
ISSN:2076-3417
-
Container-title:Applied Sciences
-
language:en
-
Short-container-title:Applied Sciences
Author:
Ashraf Muhammad AleemORCID, Tariq Hafiza Kashaf, Hu Xiao-Wen, Khan JallatORCID, Zou ZhiORCID
Abstract
Tapping panel dryness (TPD), a complex physiological syndrome associated with the rubber tree (Hevea brasiliensis Muell. Arg.), causes cessation of latex drainage upon tapping and thus threatens rubber production. Rubber tree virus 1 (RTV1) is a novel positive-sense single-stranded RNA virus from the Betaflexiviridae (genus Capillovirus), which has been established to cause TPD. MicroRNAs (miRNAs) play an important role in the interplay between viruses and host cells. In this study, we identified the rubber tree genome-encoded miRNAs and their therapeutic targets against RTV1. We applied computational algorithms to predict target binding sites of rubber tree miRNAs potentially targeting RTV1 RNA genome. Mature rubber-tree miRNAs are retrieved from the miRBase database and are used for hybridization of the RTV1 genome. A total of eleven common rubber-tree miRNAs were identified based on consensus genomic positions. The consensus of four algorithms predicted the hybridization sites of the hbr-miR396a and hbr-miR398 at common genomic loci (6676 and 1840), respectively. A miRNA-regulatory network of rubber tree was constructed with the RTV1— ORFs using Circos, is illustrated to analyze therapeutic targets. Overall, this study provides the first computational evidence of the reliable miRNA–mRNA interaction between specific rubber tree miRNAs and RTV1 genomic RNA transcript. Therefore, the predicted data offer valuable evidence for the development of RTV1-resistant rubber tree in the future. Our work suggests that similar computational host miRNA prediction strategies are warranted for identification of the miRNA targets in the other viral genomes.
Funder
Talented Young Scientist Program of China National Natural Science Foundation of China Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences
Subject
Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science
Reference49 articles.
1. Nair, K.P. (2010). The Agronomy and Economy of Important Tree Crops of the Developing World, Elsevier. 2. Rahman, A.Y.A., Usharraj, A.O., Misra, B.B., Thottathil, G.P., Jayasekaran, K., Feng, Y., Hou, S., Ong, S.Y., Ng, F.L., and Lee, L.S. (2013). Draft genome sequence of the rubber tree Hevea brasiliensis. BMC Genom., 14. 3. Go, W.Z., Chin, K.L., H’ng, P.S., Wong, M.Y., Luqman, C.A., Surendran, A., Tan, G.H., Lee, C.L., Khoo, P.S., and Kong, W.J. (2021). Virulence of Rigidoporus microporus Isolates Causing White Root Rot Disease on Rubber Trees (Hevea brasiliensis) in Malaysia. Plants, 10. 4. Huang, X., Chen, S., Yu, F., Li, Z., Zhang, Y., Wang, H., and Zhai, J. (2021). Complete Genome Sequence of a Novel Latent Tobamovirus Infecting Hevea Brasiliensis in China. Res. Sq. 5. Complete genome sequence of a novel virga-like virus infecting Hevea brasiliensis;Chen;Arch. Virol.,2022
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|